Target-site Mechanisms Involved in Annual Bluegrass(Poa annua L.) Tolerance to Fenoxaprop-P-ethyl  

早熟禾对精噁唑禾草灵耐药性的靶标酶机制研究(英文)

作　　者：王红春[1] 李俊[2,3] 吕波[4] 朱旭东[4] 娄远来[1] 董立尧[2,3] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014 [2]南京农业大学植物保护学院,江苏南京210095 [3]南京农业大学,农作物生物灾害综合治理教育部重点实验室,江苏南京210095 [4]南京农业大学理学院,江苏南京210095

出　　处：《Agricultural Science & Technology》2014年第9期1457-1465,共9页农业科学与技术（英文版）

基　　金：Supported by Special Fund for Agro-scientific Research in the Public Interest(201303022)~~

摘　　要：Annual bluegrass （Poa annua L.） was found to be tolerant to fenoxaprop- P-ethyl as well as quizalofop-P-ethyl, haloxyfop-R-methyl, clodinafop-propargyl, fluaz- ifop-P-butyl, cyhalofop-butyl, sethoxydim and tralkoxydim, whereas it was sensitive to clethodim and tepraloxydim. The acetyI-CoA carboxylase （ACCase） IC50 values of five P. annua biotypes were 10.46 to 11.98-fold higher than the susceptible Japanese foxtail （Alopecurus japonicus Steud.）. The presence of the polymorphic lie and Leu at 1 781, which the presence of Leu at 1 781 had been reported to be in- volved in the resistance of grass weeds to ACCase inhibitors, was subsequently i- dentified in the ACCase of P. annua. Furthermore, the expression level of gene that encoding P. annua ACCase was found to be approximately 4.67 to 7.37-fold higher than A. japonicus, possibly explaining the P. annua target site tolerance to fenoxaprop-P-ethyl.早熟禾对精噁唑禾草灵、精喹禾灵、高效氟吡甲禾灵、炔草酸、精吡氟禾草灵、氰氟草酯、烯禾啶和苯草酮具有耐药性．而对烯草酮和吡喃草酮敏感。早熟禾生物型的乙酰辅酶A羧化酶（ACCase）对精嗯唑禾草灵的活性显著高于敏感杂草日本看麦娘．5个早熟禾生物型的，IC50值为敏感杂草日本看麦娘，IC50值的10．46～11．98倍：早熟禾ACCase氨基酸序列1781位点存在亮氨酸／异亮氨酸的多态性现象，而亮氨酸的存在是其它禾本科杂草对ACCase抑制剂除草剂产生抗性的主要原因之一：5个早熟禾生物型的ACCase基因表达量为日本看麦娘的4．67～7.37倍。早熟禾与日本看麦娘的ACCase活性、基因序列及基因表达量差异可能是导致早熟禾对精嗯唑禾草灵具有耐药性的靶标酶机理。

关 键 词：Poa annua L.： FenoxaDroo-P-ethvl： Tolerance： AcetvI-CoA carboxylase 

分 类 号：S451.244[农业科学—植物保护]