促排卵方案对卵母细胞发育潜力及透明带的影响  

Effects of different ovulation induction protocols on developmental potential and zona pellucida of oocytes

作　　者：成俊萍[1] 何冰[1] 黄莉[1] 谭卫红[1] 薛林涛[1] 王世凯[1] 覃捷[1] 陆建柳[1] 

机构地区：[1]广西壮族自治区人民医院生殖医学与遗传中心,广西南宁530021

出　　处：《中国妇幼保健》2014年第28期4585-4588,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金项目〔81360107〕;广西科技厅面上基金项目〔2012GXNSFAA053130〕;广西卫生厅自筹课题〔Z2011459〕;广西卫生厅重点课题〔重2010043〕

摘　　要：目的:探讨标准长方案、短方案、拮抗剂方案和微刺激方案4种超排处理方法对卵母细胞发育潜力及卵母细胞透明带的影响。方法:回顾性分析170个IVF周期中患者年龄、病因相当的情况下,在标准长方案、短方案、拮抗剂方案和微刺激方案对治疗效果及卵母细胞透明带双折射值(ZB)和透明带厚度(ZT)的影响。结果:发现在不同超排方案的成熟率、受精率、多精受精率、卵裂率、优质胚胎率、附植率、妊娠率和周期取消率之间差异无统计学意义(P>0.05)。长方案组ZB值(6.32±0.37)显著低于拮抗剂方案组(10.16±0.88)和微刺激方案组(10.40±1.99),差异有统计学意义(P<0.01),短方案组MⅡ期卵母细胞ZB值也显著低于拮抗剂方案组。长方案组、拮抗剂方案组的D0ZT显著高于短方案组,长方案和拮抗剂方案组D1ZT均分别高于短方案组,差异均有统计学意义(P<0.01)。结论:卵母细胞ZB和ZT值在一定程度上能反映超促排卵方案对卵母细胞质量的影响。Objective： To explore the effects of four ovulation induction protocols （standard long protocol, short protocol, antagonist protocol and minimal stimulation protocol） on developmental potential and zona pellucida of oocytes. Methods： In the circumstance of similar age and causes, the curative effects and the impacts of standard long protocol, short protocol, antagonist protocol and minimal stimu- lation protocol on birefringence value of zona pellucida of ooeytes （ZB） and thickness of zona pellucida （ZT） were analyzed retrospectively. Results： There was no statistically significant difference in maturation rate, fertilization rate, polyspermy rate, cleavage rate, high quality embryo rate, implantation rate, pregnancy rate and cycle conceal rate between different ovulation induction protocols （ P 〉 0. 05 ） . ZB value in standard long protocol group was （6. 32 ±0. 37 ）, which was statistically significantly lower than those in antagonist protocol group and minimal stimulation protocol group （ 10. 16±0. 88, 10. 40 ± 1.99） （P 〈0. 01 ）, ZB value of stage M Ⅱ oocytes in short protocol group was significantly lower than that in antagonist protocol group. DOZT and D1ZT values in long protocol group and antagonist protocol group were statistically significantly higher than those in short protocol group （P 〈 0. 01 ） . Conclusion： ZB and ZT values of oocytes can reflect the effect of ovulation induction protocol on quality of oocvtes to a certain extent.

关 键 词：透明带 双折射 卵母细胞 体外受精 发育潜力 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]