胚胎培养液中sHLA-G的表达及其对IVF-ET妊娠结局的影响  

Expression of sHLA-G in embryo culture medium and its impact on pregnancy outcome of IVF-ET

作　　者：樊桂玲[1] 王莉[1] 王春艳[1] 韩宝生[1] 李宜学[1] 郭剑[1] 巩丽梅[1] 雷金梅[1] 郭畅[1] 金烨[1] 宁艳春[1] 吴剑华[1] 

机构地区：[1]河北省唐山市妇幼保健院生殖遗传科,063000

出　　处：《中国妇幼保健》2014年第28期4629-4631,共3页Maternal and Child Health Care of China

基　　金：河北省卫生厅医学科学研究重点课题计划项目〔20110197;20110198〕;唐山市科技局科学技术研究与发展计划项目〔12140210A〕

摘　　要：目的：探讨胚胎培养液中可溶性白细胞抗原-G（soluble leukocyte antigen-G,sHLA-G）的表达与早期胚胎发育的关系及其对IVF-ET妊娠结局的影响。方法：留取第3天移植及冷冻后的胚胎培养液,采用ELISA检测sHLA-G的表达水平。结果：1Ⅰ级胚胎与Ⅱ级胚胎培养液中sHLA-G表达水平相比较,差异无统计学意义（P〉0.05）,Ⅰ级和Ⅱ级胚胎培养液中sHLA-G表达水平均明显高于Ⅲ、Ⅳ级胚胎（P〈0.05）。2双原核不同细胞数≤4细胞、5~6细胞、7~9细胞对应胚胎培养液中sHLA-G表达水平有增高趋势,但差异无统计学意义（P〉0.05）。3妊娠组胚胎培养液中sHLA-G的表达水平显著高于非妊娠组（P〈0.05）。结论：胚胎培养液中sHLA-G的表达水平可作为预测胚胎质量及妊娠结局的参考指标之一。Objective： To explore the relationship between soluble leukocyte antigen - G （ sHLA - G） expression in embryo cul- ture medium and early embryonic development and the impact on pregnancy outcome of in vitro fertilization and embryo transfer （IVF - ET） . Methods： Embryo culture mediums were obtained on the third day of transplantation and after freezing, ELISA was used to detect the expression level of sHLA - G. Results ： There was no statistically significant difference in the expression level of sHLA - G in embryo cul- ture medium between grade I embryo and grade II embryo （ P 〉 0. 05 ） . The expression levels of sHLA - G in embryo culture medium of grade I embryo and grade Ⅱ embryo were statistically significantly higher than those of grade Ⅱ embryo and grade IV embryo （ P 〈 0. 05 ） . The expression levels of sHLA - G in embryo culture mediums of 4 blastomeres, 5 - 6 blastomeres and 7 - 9 blastomeres embryos from dipro- nucleus showed increasing trends, but there was no statistically significant difference （ P 〉 0. 05 ） . The expression level of sHLA - G in em- bryo culture medium in pregnancy group was statistically significantly higher than that in non - pregnancy group （ P 〈 0. 05 ） . Conclusion ： The expression level of sHLA - G in embryo culture medium can be used as one of predictors of embryo quality and pregnancy outcome.

关 键 词：胚胎培养液 可溶性白细胞抗原-G 胚胎质量 妊娠结局 

分 类 号：R715.5[医药卫生—妇产科学]