酸枣仁合欢方提取物中(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷含量的反相高效液相色谱法测定  

RP-HPLC determination of(-)-syringaresinol-4-O-β-D-apiofuranosyl-( 1 → 2)-β-D-glucopyranoside in Suanzaorenhehuan formula extract

作　　者：孟飞[1] 张庆伟[1] 李佩孚[1] 符敬伟[1] 乔卫[1] 

机构地区：[1]天津市临床药物关键技术重点实验室,天津医科大学药学院,天津300070

出　　处：《时珍国医国药》2014年第9期2065-2067,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81173530);天津市卫生局基金资助项目(No.02020)

摘　　要：目的 建立酸枣仁合欢方（SHF）提取物中（-）-丁香树脂酚-4-O-β-D-呋喃芹糖基-（1→2）-β-D-吡喃葡萄糖苷含量测定方法,为建立复方质量标准提供依据。方法 采用反相高效液相色谱法,色谱柱：ODS HYPERSIL C18柱（4.6 mm×250mm,5μm）;流动相：乙腈∶0.1%磷酸水（16∶84）;流速：1.0 ml·min-1;柱温：室温;检测波长：204 nm。结果 在选定的色谱条件下,酸枣仁合欢方中（-）-丁香树脂酚-4-O-β-D-呋喃芹糖基-（1→2）-β-D-吡喃葡萄糖苷在0.2~1.0μg范围内线性关系良好,直线回归方程为：Y=36 431X＋71 143,r=0.999 2,平均回收率为99.93%,RSD=1.20%。结论 该方法测定结果准确、灵敏度高、重现性好,适用于SHF提取物中（-）-丁香树脂酚-4-O-β-D-呋喃芹糖基-（1→2）-β-D-吡喃葡萄糖苷的含量测定。Objective To establish a RP-HPLC method for determination of（-）-Syringaresinol-4-O-β-D-apiofuranosyl-（ 1→2）-β-D-glucopyranoside in Suanzaoren-hehuan formula（ SHF） extract,and provide the evidence for compound quality standards. Methods Using high performance liquid chromatography（ HPLC） to measure the quality of（-）-Syringaresinol-4-O-β-D-apiofuranosyl-（ 1→2）-β-D-glucopyranoside in Suanzaorenhehuan formula. Results Using the method of HPLC to measure the quality of（-）-Syringaresinol-4-O-β-D-apiofuranosyl-（ 1→2）-β-D-glucopyranoside in SHF extract,solution concentration between 0. 2 ~ 1. 0 μg has a good linear relationship,the equations is Y = 36 431 X ＋ 71143,r = 0. 9992,and the average recovery was 99. 93%,RSD = 1. 20%. Conclusion This method is accurate with high sensitivity,good reproducibility and can be used for determination of（-）-Syringaresinol-4-O-β-D-apiofuranosyl-（ 1→2）-β-D-glucopyranoside content.

关 键 词：酸枣仁合欢方 皂苷部位 含量测定 

分 类 号：R284.2[医药卫生—中药学]