桑白皮蜜炙前后醋酸乙酯提取物指纹图谱研究  

Study on HPLC fingerprint of ethyl acetate extracts of Mori Cortex before and after baked with honey

作　　者：张会敏[1] 李群[1] 

机构地区：[1]山东省中医药研究院,山东济南250014

出　　处：《时珍国医国药》2014年第9期2147-2148,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十二五"行业专项课题(No.201107007);山东省中医药科技发展项目(No.2013-113)

摘　　要：目的 对不同产地桑白皮、蜜桑白皮醋酸乙酯提取物进行HPLC指纹图谱研究,探索桑白皮、蜜桑白皮醋酸乙酯提取物在HPLC指纹图谱中呈现的差异。方法 采用Waters 2965高效液相色谱仪进行检测,色谱柱Thermo ODS-2 HYPERSIL（250 mm×4.6 mm,5μm）,色谱条件：乙腈-0.3%磷酸水溶液梯度洗脱;柱温30℃;流速1 ml·min-1;检测波长为340 nm;进样量10μl。结果 桑白皮与由其蜜制而得蜜桑白皮醋酸乙酯提取物指纹图谱相似度为0.915~0.991,而同一产地批次不相对应的桑白皮与蜜桑白皮醋酸乙酯提取物指纹图谱相似度为0.213~0.913。结论 桑白皮蜜炙前后差异较小,而相同产地市售桑白皮与蜜桑白皮之间存在较大差异。Objective Study on ethyl acetate extract HPLC fingerprint of Mori Cortex and Mori Cortex baked with honey,to explore the differences in HPLC fingerprint of ethyl acetate extract,between Mori Cortex and Mori Cortex baked with honey. Methods Using Waters 2965 high performance liquid chromatography to detect and the chromatographic column was Thermo ODS-2HYPERSIL（ 250 mm × 4. 6 mm,5 μ m）. Chromatographic conditions acetonitrile-0. 3% phosphate aqueous gradient elution,column temperature was 30 ℃,the flow rate was 1 ml·min-1,the detection wavelength was 340 nm,the injection volume was 10μ L. Results The ethyl acetate extract fingerprint similarity is 0. 915 to 0. 991 of Mori Cortex before and after baked with honey,and The ethyl acetate extract fingerprint similarity is 0. 213 to 0. 913 of Mori Cortex and Mori Cortex baked with honey which not corresponded with each other. Conclusion The difference is small between Mori Cortex and Mori Cortex baked with honey,and there is a big difference between Mori Cortex and Mori Cortex baked with honey sold the same habitat area.

关 键 词：桑白皮 醋酸乙酯提取物 高效液相色谱 指纹图谱 

分 类 号：R283.1[医药卫生—中药学]