电针对APP/PS1转基因AD鼠Morris水迷宫学习记忆及脑皮层Aβ水平的影响  

Effect of Electro- acupuncture on Learning and Memory Behavior in Morris Water Maze and Cerebral Aβ in APP/PS1 Transgenic AD Mice

作　　者：白杨[1] 薛卫国[1] 高亮[1] 陈默[1] 辛随成[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《针灸临床杂志》2014年第8期74-77,共4页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然基金项目;编号:81273826

摘　　要：目的:探讨电针对5月龄APP/PS1转基因AD模型鼠学习记忆行为学及脑皮层Aβ水平的影响。方法:20只APP/PS1转基因阳性雄鼠随机分为模型组、电针组,每组各10只,APP/PS1转基因阴性雄鼠10只为对照组,电针组针刺"百会"、"涌泉"后接电针仪,疏密波,每次针刺持续15 min,隔日1次;对照组、模型组以相同方法用鼠袋束缚15 min,隔日1次,3组都持续5周,以Morris水迷宫检测小鼠学习记忆能力,用免疫组化方法检测脑皮层Aβ1-40、Aβ1-42的表达。结果:3组间的逃避潜伏期差异有统计学意义(P<0.05),电针组与模型组之间的差异有统计学意义(P<0.05)。免疫组化电针组Aβ1-40、Aβ1-42的表达降低。结论:电针或许通过减少Aβ的生成来提高APP/PS1双转基因小鼠的学习记忆能力。Objective： To explore the effect of electro-acupuncture on learning and memory behavior and the cerebral cortex Aβ level in five months APP /PS1 transgenic AD mice. Methods： 20 APP /PS1 transgenic positive male mice were randomly divided into a model group and an electro-acupuncture group,with 10 in each group. At the same time there were 10 APP /PS1 transgenic negative male mice in the normal control group. The electro-acupuncture group was acupunctured at＂Baihui＂and＂Yongquan＂,then was adopted the intervention of electrical needle equipment which was dilatational wave,15 min at a time,every other day at a time,treatment lasted five weeks. Finally the ability of learning and memory of all mice was tested by Morris water maze. Use immunohistochemical imaging to study the levels of cerebral Aβ in mice. Results： Escape latency between three groups was statistically significant（ P〈0. 05）; the difference between the model group and the electro-acupuncture group was statistically significant（ P〈0. 05）. Expressions of Aβ1-40 and Aβ1-42 of the electro-acupuncture group were lower than those in the model group. Conclusion： Electro-acupuncture can probably improve learning and memory ability of APP /PS1 double transgenic mice through reducing the level of Aβ.

关 键 词：电针 APP PS1 MORRIS水迷宫 学习记忆 Aβ水平 

分 类 号：R245.97[医药卫生—针灸推拿学]