红霉素对支气管哮喘儿童Th1／Th2细胞及T淋巴细胞凋亡的调控研究  

作　　者：于磊[1] 王欢欢[1] 秦璞[1] 冯金环[1] 

机构地区：[1]青岛大学医学院,山东青岛266021

出　　处：《特别健康（下）》2014年第1期277-278,共2页SPECIAL HEALTH

摘　　要：目的探讨红霉素对支气管哮喘儿童TH1／TH2细胞及T淋巴细胞凋亡的调控。方法按哮喘诊断标准选取哮喘患儿74例，入选后留取血液标本，设为对照组，之后给予红霉素口服，共30天，留取血液标本，设为治疗组，采用荧光单细胞内染色法检测经红霉素治疗前后Th1／Th2细胞活性变化，用双抗体夹心ABC—ELISA法检测治疗前后血清sFas、sFasL水平，用吖啶橙-溴化乙锭染色法检测治疗前后T淋巴细胞凋亡率，结果与对照组比较，并用统计学分析。结果治疗组Th1、Th1／Th2较对照组显著下降（P〈0．01）；sFas、sFasL、Th2较对照组明显升高（P〈0．01）；培养0h、48h淋巴细胞凋亡率显著上升（P〈0．01）。结论红霉素对哮喘患儿Th细胞有明显的调控作用，促进Th细胞向Th1转化；调节T细胞亚群的紊乱，对Fas介导的T淋巴细胞的凋亡有调节作用，降低Fas介导T淋巴细胞（Th1）的凋亡。Objective： To investigate the effects of erythromycin on the regulation of the Th1/Th2 cells and T lymphocyte apoptosis in peripheral blood mononu- clear cells（PBMCs） of bronchial asthmatic children. Methods ： Seventy - four cases were included according to the diagnostic criteria of asthma children, peripheral blood mononuclear cells（PBMCs） before and after erythromycin treatment for thirty days were isolated from venous blood, Levels of Th1 ,Th1/Th2 of PBMCs were de- termined by single - cell fluorescence staining method ; Levels of sFas and sFasL in serum of asthmatic children before and after the treatment was examined with sandwich ELISA methods; Rates of T lymphocyte apoptosis incubated for Oh and 48h before and after the treatment were detected with acridone acetamiprid orange -brominated b ingot dyeing method. Statistical analysis was performed. Results： After erythromycin treatment, the levels of Th1, Th1/Th2 were significantly de- creased than before treatment （ p 〈 0.01 ） ; the Th2 and serum sFas, sFasL levels were significantly higher than before （ p 〈 0.01 ） ; lymphocyte apoptosis rates incubated for Oh, 48h were significantly increased than before （ P 〈 0, 01 ）. Conclusions ： Erythromycin may reduce the Fas mediated apoptosis of T lymphocytes （ Th1 ） , promote the production of Th1 in PBMC, and restore the balance of Th1/Th2 existed in asthmatic children. Children with asthma could benefit from the use of cefu roxime.

关 键 词：红霉素 支气管哮喘 THL TH2 细胞凋亡 调控 

分 类 号：R725.6[医药卫生—儿科]