铁皮石斛中多糖和甘露糖含量测定方法的改进及与齿瓣石斛的比较研究  

Improvement in Determination for Polysaccharide and Mannose in Dendrobii Officinalis Caulis and Its Comparation with Dendrobii Devoniani Caulis

作　　者：甘小娜[1,2] 徐英[1,2] 徐红[1,2] 刘家保 杨莉[1,2] 王峥涛[1,2] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室中药新资源与质量标准综合评价国家中医药管理局重点研究室上海市复方中药重点实验室,上海201203 [2]上海中药标准化研究中心,上海201203 [3]云南省龙陵县林业局,保山678300

出　　处：《中国药品标准》2014年第4期276-279,共4页Drug Standards of China

基　　金：国家自然科学基金优秀青年基金(81222053)

摘　　要：目的：改进铁皮石斛中多糖和甘露糖的含量测定方法并对铁皮石斛与齿瓣石斛中的多糖和甘露糖含量进行分析比较。方法：采用硫酸-苯酚法测定石斛中多糖的含量；采用高效液相色谱法测定石斛中甘露糖的含量。结果：多糖在2.54～12.87 mg· L-1范围线性关系良好（r＝0.9990），平均回收率为99.02％，RSD为2.6％（n＝6）；甘露糖的平均回收率为101.5％，RSD为1.7％（n＝9）。6批铁皮石斛药材多糖平均含量为45.94％，甘露糖平均含量为32.33％；9批齿瓣石斛药材多糖平均含量为45.12％，甘露糖平均含量为37.88％。结论：所建方法操作比较简单，准确可靠，重现性好，可用于控制铁皮石斛的质量。铁皮石斛与齿瓣石斛多糖含量相当，齿瓣石斛甘露糖含量高于铁皮石斛。Objective：To improve the methods for determining polysaccharide and mannose in Dendrobii Officinalis Caulis and com -pare the contents of polysaccharide and mannose in Dendrobii Officinalis Caulis and Dendrobii Devoniani Caulis , respectively.Meth-ods：Sulfuric acid-phenol method was adopted to determine the content of polysaccharide and HPLC was used to determine the content of mannose.Results：The calibration curve of polysaccharide was linear in the range of 2.54-12.87 mg· L-1 （ r=0.9990 ） the aver-age recovery （ n=6 ） of polysaccharide was 99.02% （ RSD =2.6%）.The average recovery （ n =9 ） of mannose was 101.45%（RSD=1.7%）.Conclusion：The methods are convenient , accurate, reliable and reproducible for quality control of Dendrobii Offici-nalis Caulis.Dendrobii Officinalis Caulis and Dendrobii Devoniani Caulis are similar in the content of polysaccharide ; the content of mannose in Dendrobii Devoniani Caulis is higher than Dendrobii Officinalis Caulis .

关 键 词：铁皮石斛 多糖 甘露糖 含量测定 齿瓣石斛 

分 类 号：R921.2[医药卫生—药学]