中国人群MNS血型低频抗原Vr,Mta,Ria,Cla,Nya,Or,Osa,SD和Mit基因座多态性调查研究  

Investigation and Study of the Low Frequency Antigens＇ Locus Polymorphism about the MNS Blood Group＇s Vr,Mta,Ria,Nya,Or,Osa, SD and Mit in Chinese Population

作　　者：梁延连[1] 苏宇清[1] 吴凡[1] 周丹[1] 李大成[1] 张印则[1] 

机构地区：[1]深圳市血液中心,广东深圳518035

出　　处：《现代检验医学杂志》2014年第4期46-49,共4页Journal of Modern Laboratory Medicine

基　　金：深圳市科技资助项目（立项编号：201202142）.

摘　　要：目的 调查中国人群MNS血型低频抗原Vr,Mta,Rr,CP,Nva,Or,Osa,SD和Mit基因座碱基变异点,为MNS血型低频抗原的研究、临床输血与免疫血液病的诊断与治疗打下基础.方法 随机选取深圳市血液中心无偿献血人群500人份提取血样DNA,应用自主设计的引物直接测序分析GYPA的A3,GYPB的B4碱基序列,分析中国人群MNS血型低频抗原Vr,Mta,Rr,CP,Nya,Or,Osa,SD和Mit基因座的突变位置.结果 从GYPA的A3,GYPB的B4中分析了MNS血型低频抗原Vr,Mta,Rr,CP,Nya,Or,Osa,SD和Mit基因突变位点,鉴定了相关抗原基因的变异点分别为：GYPA的A3中197,230,226,138,148和217;GYPB的B4中173和161.结论 MNS血型抗原Vr,Mta,Rr,CP,Nva,Or,Osa,SD和Mit虽为低频抗原,但在免疫条件下同样会引起输血反应与严重的新生儿溶血病（HDN）,使用基因检测的方法可以解决低频抗体血清的短缺,为临床输血与免疫性疾病诊断以及人类群体遗传学研究打下基础.Objective To investigae the low frequency antigens＇ locus Polymorphism about the MNS blood group of the Vr,Mta,Ria,Nya,Or,Osa,SD and Mit in Chinese population to lay the foundation for the basis of the low frequencys about the MNS blood group antigens and immune diseases of the blood transfusion.Methods In 500 blood donors as samples and extraction of DNA from blood,according to the GenBank NG-007470 and NG-007483 gene sequence as the basis to study the A3 of the GYPA and the B4 of the GYPB.The mutation could analyse the low frequency antigen about the MNS blood group of the Vr,Mta,Ria,Nya,Or,Osa,SD and Mit in Chinese population.Results The MNS blood group antigens of the Vr,Mta,Ria,Nya,Or,Osa,SD and Mit gene mutations were analysed and identified from the A3 of the GYPA and the B4 of the GYPB.The gene locis were 197,230,226,138,148 and 217 in the A3 of the GYPA.The gene locis were 173 and 161 in the B4 of the GYPB.Conclusion Although the Vr,Mta,Ria,Nya,Or,Osa,SD and Mit are the low frequency antigens about the MNS blood group but also may cause transfusion reactions and severe HDN in immune condition.The gene detection method can solve the problem about the rare antibody serum.The study can lay the foundation for clinical blood transfusion,the diagnosis of autoimmune disease and the study of human population genetics.

关 键 词：中国人群 MNS血型系统 低频抗原 基因多态性 

分 类 号：R457.11[医药卫生—治疗学] Q786[医药卫生—临床医学]