PPAR-δ在高压氧诱导大鼠肺组织损伤中的作用  

Effect of PPAR-δ on lung injury of rats induced by hyperbaric oxygen exposure

作　　者：王芳芳[1] 方以群[1] 包晓辰[1] 马骏[1] 攸璞[1] 张师[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《军事医学》2014年第8期591-593,601,共4页Military Medical Sciences

基　　金：海军后勤部科研基金项目(12A107);海军医学研究所所基金项目(12HY11);总后"十二五"重点科研课题(BWS11J053;BHJ12J003)

摘　　要：目的研究过氧化物酶增殖物激活受体-δ(PPAR-δ)在高压氧(hyperbaric oxygen,HBO2)诱导大鼠肺组织损伤中的作用。方法 60只雄性SD大鼠随机分为6组:air+vehicle,air+GW0742,air+GSK0660,HBO2+vehicle,HBO2+GW0742,HBO2+GSK0660,每组10只,分别暴露于常压空气或2.3 ATA纯氧中8 h。分别于进舱前1、6、12 h给予大鼠腹腔注射溶于10%二甲亚砜(DMSO)中的生理盐水(0.3 ml)、PPAR-δ激动剂GW0742(0.3 mg/kg,ip)、PPAR-δ阻滞剂GSK0660(1 mg/kg,ip)。出舱后30 min检测肺组织湿干比、肺泡灌洗液蛋白含量、肺组织病理形态变化。结果与结论与空气对照组比较,HBO2+GW0742组大鼠肺组织湿干比、支气管肺泡灌洗液蛋白含量、组织病理学损伤改变减轻,而GSK0660应用能加重肺组织损伤。PPAR-δ激活对高压氧诱导的肺型氧中毒具有保护作用。Objective To investigate the effect of PPAR-δ on the lung injury of rats induced by hyperbaric oxygen （HBO2） exposure.Methods Sixty male Sprague-Dawley rats were randomly divided into six groups：air＋vehicle, air＋GW0742, and air＋GSK0660, HBO2 ＋vehicle, HBO2 ＋GW0742, HBO2 ＋GSK0660.Lung injury was induced in rats by HBO2exposure （2.3 ATA, 100%O2, 8 h）.Rats were injected with vehicle[10%DMSO in 0.3 ml NaCl 0.9%（v/v）] or GW0742 （0.3 mg/kg, ip） or GSK0660 （1 mg/kg, ip） at 1, 6 and 12 hours before either air or oxygen exposure .Protein levels in the bronchoalveolar lavage fluid （ BALF） , wet/dry ratio of the lung and the pathological changes in the lung tissue were detected 30 min after rats′egress.Results and Conclusion For the HBO2 ＋GW0742 group, the protein levels in BALF, the wet/dry ratio of the lung and the pathological changes in lung tissues all significantly decreased compared with those of the air group .These changes in HBO 2 ＋GSK0660 group tended to increase the level of lung injury .PPAR-δhas a protective effect on pulmonary oxygen toxicity induced by HBO 2 .

关 键 词：过氧化物酶增殖物激活受体-δ 高压氧 肺型氧中毒 

分 类 号：R459.6[医药卫生—治疗学] R595.1[医药卫生—临床医学]