“Y”型棉涤线微流控分析通道测定新方法研究  

A Novel Method Based on Y-Shaped Cotton-Polyester Thread Microfluidic Channel

作　　者：王璐[1] 石彦茹 闫宏涛[1] 

机构地区：[1]西北大学化学与材料科学学院,陕西西安710069

出　　处：《光谱学与光谱分析》2014年第8期2234-2237,共4页Spectroscopy and Spectral Analysis

基　　金：国家科技支撑计划项目(2010BAK67B12)资助

摘　　要：基于棉涤线的毛细作用，构建成“Y”型微流控分析通道，自行研制了一种可调控试样流速的微流控分析通道装置，实现了待测液和显色剂同时进样，研究建立了一种“Y”型棉涤线微流控分析通道分析测定新方法。进行了分光光光度法与Scan-Adobe Photoshop软件处理两种检测方法的比较，结果表明光度法检出限低；Scan-Adobe Photoshop软件处理法操作简捷，分析速度快，样品用量少。应用于亚硝酸根的分析测定，两种检测方法的线性范围和检出限分别为1．0～70μmol＆#183;L-1，0．66μmol＆#183;L-1（光度法）；50～450和45．10μmol＆#183;L-1（Scan-Adobe Photoshop软件处理法）。回收率在96．7％～104．0％之间。该微流控通道分析方法成本低廉，分析速度快，对土壤和水样中亚硝酸根进行分析测定，结果满意。A novel method based on Y-shaped microfluidic channel was firstly proposed in this study.The microfluidic channel was made of two cotton-polyester threads based on the capillary effect of cotton-polyester threads for the determination solu-tions.A special device was developed to fix the Y-shaped microfluidic channel by ourselves,through which the length and the tilt angle of the channel can be adjusted as requested.The spectrophotometry was compared with Scan-Adobe Photoshop software processing method.The former had a lower detection limit while the latter showed advantages in both convenience and fast oper-ations and lower amount of samples.The proposed method was applied to the determination of nitrite.The linear ranges and de-tection limits are 1. 0~70μmol＆#183;L-1 ,0. 66μmol＆#183;L-1 （spectrophotometry）and 50~450μmol＆#183;L-1 ,45. 10μmol＆#183;L-1 （Scan-Adobe Photoshop software processing method）respectively.This method has been successfully used to the determination of ni-trite in soil samples and moat water with recoveries between 96. 7% and 104%.It was proved that the proposed method was a low-cost,rapid and convenient analytical method with extensive application prospect.

关 键 词：棉涤线 毛细作用 微流控分析 亚硝酸根测定 

分 类 号：O657[理学—分析化学]