马里兰烟病毒病症状类型、毒源种类及病毒侵染构型研究  

Classification of Virus Disease Symptoms,Kinds of Virus Species and Types of Infection of Virus on Maryland Tobacco

作　　者：许汝冰 李锡宏 雎韡 文光红 刘圣高 黎妍妍 侯明生[2] 

机构地区：[1]湖北省烟草科研所,武汉430030 [2]华中农业大学植物科学技术学院,武汉430070 [3]宜昌市烟草公司,湖北宜昌443000

出　　处：《中国烟草科学》2014年第4期85-87,共3页Chinese Tobacco Science

基　　金：国家烟草专卖局项目"全国烟草有害生物调查研究"(110200902065);湖北省烟草专卖局重大攻关项目"湖北烟草有害生物调查及综防预警网络平台体系建设"(027Y2010-32)

摘　　要：湖北五峰县是中国唯一的马里兰烟种植区。2011年在湖北马里兰烟产区进行病毒病发生情况大田调查,发现主要有花叶、畸形、脉坏死、蚀刻等4种症状类型,并采用指示植物法对主要症状类型所含病毒的种类进行了鉴定;对采集的57份病毒病样本采用DAS-ELISA进行病毒种类的鉴定。结果表明,发生在马里兰烟上的烟草病毒种类包括TMV、CMV、PVX、PVY和TEV,TMV检出率最高,PVY次之;以2种或2种以上病毒复合侵染方式为主,其中TMV+PVY、TMV+CMV+PVY共存方式较为常见。Wufengcountyin Hubei province is the only Maryland tobacco plantingarea in China. Maryland tobaccovirus diseases wereinvestigatedtherein 2011.The symptom types ofvirusdiseases could be divided into4mainkinds, including mosaic, distortion, vein necrosis,etching, which were identified through indication plants for virus species.DAS-ELISA was applied to identify the virus speciesto the collected 57 samples. It demonstrated that the virus species which infected Maryland tobacco included TMV, CMV, PVX, PVY and TEV. The detection rate of TMV was maximal among all the tested samples, and PVY took the second place. Meanwhileit commonlyoccurredthattwo and more kinds of virus co-existed, and TMV＋PVY, TMV＋CMV＋PVY were the main patterns.

关 键 词：马里兰烟 烟草病毒病 症状 病毒种类鉴定 病毒侵染构型 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]