TRPV1与μ-阿片受体在胃投射脊髓背根神经节中的共存表达及其临床意义  

The coexistence expression of TRPV1 and μ-opoid receptor in spinal dorsal root ganglion projected to stomach and its clinical significance

作　　者：蒋群[1] 屠伟峰[2] 徐少群[1] 赵高峰[1] 郄文斌[2] 李国才[1] 

机构地区：[1]广东省中医院麻醉科,广州市510120 [2]广州军区广州总医院全军临床麻醉中心,广州市510010

出　　处：《实用医学杂志》2014年第18期2876-2879,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81272141);广东省自然科学基金(编号:S2011010003373);江苏省高校重点实验室开放课题(编号:KJS09002)

摘　　要：目的:观察瞬时受体电位离子通道(TRPV1)与μ-阿片受体在胃调控脊髓背根神经节中的共存表达,探讨其在急性胃黏膜损伤治疗中的临床意义。方法:选取雄性Wistar大鼠分三组,正常对照组(NC组)、浸水束缚应激(WIRS)组(WIRS组)和舒芬太尼预处理组(SP组)。采用经典WIRS方法复制急性胃黏膜损伤(AGML)模型,于应激6 h后观察大体胃黏膜损伤程度,并记录溃疡指数(GI),并采用免疫荧光方法观察TRPV1与μ-阿片受体在胸段DRG神经元中的表达情况,并通过ELASA法测定各组大鼠胃黏膜组织中CGRP含量。结果:TRPV1与μ-阿片受体在胸段胃投射DRG神经元上共存表达,且AGML大鼠胃黏膜GI和CGRP含量都明显增高,而舒芬太尼预处理虽使CGRP含量更高,GI较WIRS组显现非常显著下降。结论:TRPV1参与了急性胃黏膜损伤的保护机制,激活μ-阿片受体可活化TRPV1释放CGRP从而发挥胃黏膜保护作用。Objective To observe the coexistence expression of TRPV1 and μ-opoid receptorin spinaldorsal root ganglion （DRG） projected to stomach, and to investigate the relationship between TRPV1 andp.-opoid receptorand its clinical significance in rats with acute gastric mucosal lesion induced by water immersionand restraint stress. Methods FortyWistar rats were randomly assigned to 3 groups, including normal controlgroup（group NC, n = 10）, WIRS group （group WIRS, n = 20）, and sufentanil pretreatment group （group SP,n = 10）. A rat model of gsatric mucosal lesion was induced by WIRS. 6 hours after WIRS treatment, gastrictissues were excised and microscopically observed; ulcer index （GI） was noted. The coexistence expression ofTRPV1 and μ-opioid receptor in DRG neurons was detected by immunofluorescence assay, and the levels ofCGRP was measured by ELASA. Results As compared withgroup WIRS, the degree of gastric injury wasobviously relieved in group SF. Coexistence of TRPV1 and μ-opioid receptor was detected in thoracic DRGneurons projected to stomach; the CGRP level was higher in group WIRS than in group NC. ConclusionsTRPV1isinvolved in protection of acute gastric mucosal lesion. Activation of Ix-opioid receptor can induce TRPV1 torelease CGRP, resulting in protection of gastric mucosa.

关 键 词：Μ-阿片受体 TRPV1 CGRP 舒芬太尼 急性胃黏膜损伤 

分 类 号：R614[医药卫生—麻醉学]