Ad-BMP-2、Ad-TGF-β3转染BMSCs复合DBM构建软骨修复猪关节软骨缺损的研究  

Experimental study of cartilage defect repair using bone mesenchymal stem cells transfer of BMP-2 and TGF-β3 gene mediated by adenovirus vector combining with deminerized bone matrix in pig

作　　者：王鑫[1] 李彦林[1] 金耀峰 陈建明[3] 王慧建[1] 何川[1] 曹树海[1] 赵沣凯 

机构地区：[1]昆明医科大学第一附属医院运动医学科,650032 [2]浙江省嘉兴市人民医院骨科,410000 [3]湖南省第二人民医院骨科,410000

出　　处：《实用医学杂志》2014年第18期2880-2882,共3页The Journal of Practical Medicine

基　　金：教育部博士点基金资助项目(编号:20115317110001);云南省联合专项基金资助项目(编号:2010FB);云南省医学学科带头人培养基金(编号:D-201207)

摘　　要：目的:观察腺病毒介导的骨形态发生蛋白-2(Ad-BMP-2)和转化生长因子-β3(Ad-TGF-β3)双基因转染骨髓间充质干细胞(BMSCs),复合脱钙骨基质(DBM)构建新型组织工程软骨修复猪关节软骨缺损的效果。方法:成年滇南小耳猪8头,16膝,制作32个全层软骨缺损模型。A组植入双基因转染的BMSCs+DBM,B组植入未经转染的BMSCs+DBM,C组单纯植入DBM,D组为空白对照;术后2、4、8、12周取材进行形态学和组织学观察。结果:术后12周,A组缺损区被软骨样组织修复,HE染色显示有典型的透明软骨结构,D组软骨缺损主要由纤维组织充填。O′driscoll评分A、B、C、D组组间差异有统计学意义(P<0.05)。结论:Ad-BMP-2和Ad-TGF-β3双基因转染的猪BMSCs复合DBM构建的组织工程软骨可有效修复关节软骨缺损。Objective To explore the repair result of full-thickness cartilage defects in diannan small-earpig by bone mesenchymal stem cells （BMSCs） transferred with both transforming growth factor-β3 （TGF-133） andbone morphogenetic protein-2 （BMP-2） gene mediated by adenovirus vector and combined with deminerized bonematrix （DBM）. Methods 32 full-thickness defects from 16 knees of 8 pigs were randomly divided into 4 groupsin the experiments. In group A, the animals＇ lateral femoral condyle of right knee joint was repaired with DBMand BMSC infected with both Ad-TGF-β3 and Ad-BMP-2. In group B, the medial femoral condyle of right kneejoint was repaired with DBM and BMSC without infection. In group C, the lateral femoral condyle of left kneejoint was repaired with DBM. And the group D is control group. Morphology and histology were observed 2, 4, 8and 12 weeks after operation. Results 12 weeks after operation, the whole defects were repaired in group A,HE staining showed typical cartilaginous structure in the repaired area. In group D, defects were not repaired butfilled with fibrous tissue. The O＇driscoll scores were 15.65±0.11 （group A）, 11.33± 0.22 （group B）, 6.13 ±0.15 （group C） and 5.08 ± 0.15 （group D）. There was significant difference among the groups （P 〈 0.05）.Conclusions The new type of tissue engineering scaffold that DBM combined with BMSCs transfected with bothAd-BMP-2 and Ad-TGF-β3 could induce cartilage regeneration and repair the defects.

关 键 词：基因转染 组织工程 脱钙骨基质 软骨缺损 

分 类 号：R681[医药卫生—骨科学]