失血条件下氰类毒剂中毒对大鼠肺换气功能及磷脂含量的影响  

The effects of cyanide compounds intoxication and hemorrhagic shock on the function of rats lung

作　　者：刘辉[1] 燕颖军[1] 尹芳秋[1] 贾庆军[1] 王晓红[1] 边巍[1] 

机构地区：[1]中国人民解放军白求恩医务士官学校,河北石家庄050081

出　　处：《工业卫生与职业病》2014年第5期342-344,共3页Industrial Health and Occupational Diseases

基　　金：全军医学科技"十二五"科研基金项目(CWS11J156)

摘　　要：目的观察失血条件下氰类毒剂中毒对大鼠肺换气功能及磷脂含量的影响。方法将健康成年SD大鼠随机分为假手术组、失血性休克组、氰化钾中毒组和失血性休克复合氰化钾中毒组,每组6只动物。各实验组设15、30、60和120min 4个时相点,检测大鼠肺换气功能的变化,采用伊文思蓝(Evan’s blue,EB)染色观察肺血管通透性的改变以及定磷法测定肺组织中磷脂总量(total phospholipids,TPL)。结果与氰化钾中毒组比较,失血条件下氰化钾中毒大鼠肺泡动脉血氧分压差(PAaDO2)和呼吸指数(RI)不同程度升高,大鼠肺部血管通透性增强以及肺磷脂含量明显降低,差异有统计学意义(P<0.05)。结论战(创)伤失血明显加重氰类毒剂对肺功能的损伤效应。Objective To study the effects of cyanide compounds intoxication and hemorrhagic shock on pulmonary gas exchange and phospholipids contents of the rats.Methods SD rats were randomized equally into sham operation group（SO）,hemorrhagic shock group（HS）,potassium cyanide intoxication group（KCN）and hemorrhagic shock combined potassium cyanide intoxication group（HS＋KCN）.Blood samples used for blood-gas analysis were taken at the time point of 15 min,30min,60 min and 120 min after cessation of exposure,Evan＇s blue（EB）in lung tissues was examined by spectrophotometric method and the content of phospholipids was assayed with a biochemistry method.Results Compared with KCN group,PAaDO2 and RI were obviously increased in（HS＋KCN）group.Combined hemorrhagic shock and KCN intoxication produced a significant increasing effect on EB content and decreasing effect on phospholipids content.Conclusions Hemorrhagic shock and cyanide intoxication may induce a combined injury effect on rats lung.

关 键 词：失血 氰化钾 磷脂 肺换气功能 

分 类 号：R342.0[医药卫生—基础医学]