战(创)伤失血复合氰化物中毒对大鼠心功能的影响  

Effects of hemorrhagic shock complicated with cyanide poisoning on heart function of rat

作　　者：刘辉[1] 燕颖军[1] 尹芳秋[1] 贾庆军[1] 白小嘉[1] 边巍[1] 赵岚[1] 

机构地区：[1]中国人民解放军白求恩医务士官学校战场救护与防护教研室,河北石家庄050081

出　　处：《工业卫生与职业病》2014年第5期345-348,351,共5页Industrial Health and Occupational Diseases

基　　金：全军医学科技"十二五"科研基金项目(CWS11J156)

摘　　要：目的观察战(创)伤失血复合氰化物中毒对大鼠心功能的影响。方法将成年SD大鼠随机分为假手术组、失血性休克组、氰化钾中毒组和失血性休克复合氰化钾中毒组,观察各组大鼠心肌线粒体超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)活力的变化,采用生理信号采集系统记录大鼠心率(HR)、左心室收缩压峰值(mLVSP)和左心室压力上升最大速率(+dp/dtmax),采用生化分析法检测大鼠心肌酶谱天冬氨酸转氨酶、乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶(AST、LDH、CK和CKMB)的活力变化。结果与氰化钾中毒组比较,失血复合氰化钾中毒组大鼠心肌中线粒体SOD、GSH-Px活力明显降低,MDA含量升高;大鼠心脏HR、mLVSP、+dp/dtmax和心肌酶谱活力变化明显异常。结论战(创)伤失血条件下氰化物对大鼠心脏功能的影响更为严重,需要进一步探索相应的防治措施。Objective To investigate effects of hemorrhagic shock complicated with cyanide poisoning on heart function of rat.Methods SD rats were randomly and equally divided into sham operation group（SO）,hemorrhagic shock group（HS）,potassium cyanide poisoning group（KCN）and hemorrhagic shock complicated with potassium cyanide poisoning group（HS＋KCN）.The activities of SOD,GSH-Px and the content of MDA were assayed in rat myocardium mitochondria.The left ventricular systolic pressure（mLVSP）,＋dp/dtmaxand heart rate（HR）were recorded by physiological function signal processing system.The arterial blood was taken for myocardium zymogram detection（AST,LDH,CK,and CK-MB）.Results Compared with KCN group,SOD,GSH-Px activities and MDA content obviously decreased,the mLVSP,＋ dp/dtmax and HR changed obviously in the rats of HS＋ KCH group,meanwhile,the myocardium zymogram of rats showed significant changes accordingly.Conclusions Under the condition of hemorrhagic shock,complicated cyanide poisoning would impair the cardiac function more severely,further study on effective measures for prevention and treatment is urgently required.

关 键 词：失血 氰化钾 心功能 心肌酶谱 联合作用 

分 类 号：R342.0[医药卫生—基础医学]