艾燃烧生成物对快速老化模型小鼠P8大脑单胺类神经递质含量的影响  

Effects of folium artemisiae argyi burning products on monoamine neurotransmitters contents in brain of senescence-accelerated mouse P8

作　　者：孟笑男[1] 许焕芳[2] 崔莹雪[3] 刘平[4] 赵百孝[3] 

机构地区：[1]北京中医药大学附属护国寺中医医院,北京100035 [2]中国中医科学院广安门医院,北京100053 [3]北京中医药大学,北京100029 [4]北京电力医院,北京100073

出　　处：《中华中医药杂志》2014年第9期2761-2763,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2009CB522906);高等学校博士学科点专项科研基金(No.20090013110004)~~

摘　　要：目的:观察艾燃烧生成物即艾烟对快速老化模型小鼠(SAM)P8大脑内单胺类神经递质含量变化的影响。方法:本实验选取6月龄雄性SAM50只,其中SAMR1系10只为空白对照组,SAMP8系40只平均随机分为模型对照组、单纯艾烟组、无烟艾灸组和常规艾灸组。常规艾灸组小鼠艾灸关元穴;无烟艾灸组则在艾灸小鼠关元穴时立即将艾烟抽除;单纯艾烟组小鼠艾烟干预;空白对照组和模型对照组小鼠空白抓取。实验结束后采取酶联免疫吸附法(ELISA)检测其大脑内单胺类神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)和多巴胺(DA)含量。结果:单纯艾烟组和无烟艾灸组其脑内5-HT的含量,单纯艾烟组、常规艾灸组及无烟艾灸组其脑内DA和HE的含量均显著高于模型对照组(P<0.05,P<0.01),而三组间其含量差异无统计学意义。结论:适当施灸时,一定浓度范围之内的艾燃烧生成物可以治疗性提高SAMP8小鼠脑内单胺类神经递质的含量。Objective： To observe effect of folium artemisiae argyi burning products （moxa smoke） on contents of brain monoamine neurotransmitter of SAMP8 mice. Methods： 50 SAMP8 mice were chosen, of which 10 SAMR1 mice consisted control group, and the residual 40 SAMP8 mice were randomly divided into model group, moxa smoke group, routine moxibustion group and non-smoke moxibustion group, with 10 mice in each group. Mice in routine moxibustion group were given moxibustion on Guanyuan point. Mice in non-smoke moxibustion group were also given moxibustion on Guanyuan point, but the moxa smoke was dispelled. Mice in moxa smoke group were just fumed with moxa smoke. Mice in control group and model group were just captured by the same methods with other groups. The contents of 5-HT, NE and DA were tested by ELISA. Results： Compared with control group, there was a remarkable drop on contents of brain neurotransmitters in model group. Compared with model group, contents of DA and NE in routine moxibustion group, non-smoke moxibustion group and moxa smoke group, contents of 5-HT in non-smoke moxibustion group and moxa smoke group, were all increased （P〈0.05, P〈0.01）, but there was no statistical difference between 3 intervention groups. Conclusion： Under some circumstance, folium artemisiae argyi burning products with proper concentration are curative, which can increase contents of brain monoamine of SAMP8

关 键 词：艾燃烧生成物 快速老化模型小鼠 单胺类神经递质 

分 类 号：R245[医药卫生—针灸推拿学]