复方浙贝颗粒药物血清联合阿霉素对P388细胞增殖抑制作用初探  

Inhibition effect of serum containing medicine of Compound Zhebei Granule combined with adriamycin on proliferation of P388 cells

作　　者：孙叙敏 陈信义[2] 

机构地区：[1]煤炭总医院,北京100028 [2]北京中医药大学东直门医院,北京100700

出　　处：《中华中医药杂志》2014年第9期2764-2767,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.30672691)~~

摘　　要：目的:探索复方浙贝颗粒(CZBG)药物血清联合阿霉素(ADR)在体外对P388细胞的增殖抑制作用及机制。方法:1制备高、中、低剂量CZBG药物血清,以不同血清给药浓度作用于小鼠急性淋巴细胞白血病P388细胞株,MTT法观察CZBG药物血清对P388的抑制作用;2以不同剂量及浓度的CZBG药物血清联合ADR与单用ADR比较,分析联合用药后对P388细胞的增殖抑制影响;3流式细胞仪(FCM)定量检测实验各组P388细胞内阿霉素荧光值;Annexin-V/PI双染法检测P388细胞早期凋亡率。结果:1CZBG药物血清组及空白血清组在5%、10%及20%浓度时随着浓度增加对P388细胞株逐渐显示出抑增殖趋向,未发现两者间有统计学差异。CZBG药物血清高、中、低剂量在20%血清给药浓度时配伍不同浓度的ADR作用48h有增强ADR效应的趋势,剂量越高,增效作用越明显。其中CZBG高剂量联合ADR 0.04μg/mL及0.2μg/mL组与单用ADR组比较有显著性差异(t值分别为3.38,7.17,P<0.05);CZBG高、中、低剂量联合ADR用药后ADR IC50由0.46μg/mL分别降至0.05、0.30、0.30μg/mL;2经ADR作用后,不同剂量组的P388细胞内ADR荧光强度明显高于空白血清组。低剂量组最高,中,高剂量组次之。中剂量组早期凋亡率最高,为22.6%,高剂量组次之,为18.2%,低剂量组为16.4%,空白血清组为15.7%。结论:复方浙贝药物血清有增强阿霉素对P388细胞的增殖抑制趋向,可能与增加细胞内阿霉素含量,促进细胞凋亡有关。Objective： To explore the anti-proliferative effect and mechanism of serum containing medicine of Compound Zhebei Granule （CZBG） combined with adriamycin （ADR） on proliferation of P388 cells in vitro. Methods： The high, medium and low doses of CZBG were prepared. The P388 leukemia mice cells were treated with different doses of CZBG. The inhibition effect of CZBG was tested by MTT. P388 leukemia mice cells were treated with different doses of CZBG combined with ADR and ADR single, and the inhibition effect of these two treatments was compared. Flow cytometer （FCM） was used to test the intracellular fluorescence value of ADR and early apoptosis rate of cells. Results： The inhibiting effect of CZBG on P388 cells was increased with the increase of medicine concentration, but there was no significant difference. Treating p388 cells with drug serum of high, medium and low dose of CZBG combined with different concentrations of ADR with 48h could enhance the effect of ADR, and more obvious effects with the increase of dosage. The differences between high doses of CZBG combined with 0.04 and 0.2lag~ mL ADR and ADR alone were significant （t=-3.38, 7.17, P〈0.05）. After the treatment of high, medium and low doses of CZBG combined with ADR, ICs0 decreased from 0.461ag/mL to 0.05, 0.30 and 0.30tag/mL respectively. The results of FCM showed that the ADR fluorescence intensity in P388 cells was higher than that in control group. Early apoptosis rate of medium dose group was 22.6%, which was the highest. High dose group was the second, with 18.2%. Low dose group was 16.4%, and the control groupwas 15.7%. Conclusion： CZBG could enhance the inhibition effect of ADR on P388 cells by increasing the intracellular ADR content and promoting the apoptosis.

关 键 词：复方浙贝颗粒 MTT法 P388细胞 血清药理学 阿霉素 细胞增殖 

分 类 号：R285[医药卫生—中药学]