不同中医证型鼻咽癌原发病瘤灶组织差异表达蛋白  

Differential expression proteins in the primary focus of nasopharyngeal carcinoma with different patterns in terms of TCM

作　　者：何迎春[1,2] 陈艳[1] 廖端芳[1,2] 何兰[1] 林丽美[1] 范婧莹[1] 卢芳国[1] 田道法[3] 彭建辉[4] 

机构地区：[1]湖南中医药大学,长沙410208 [2]南华大学,衡阳421001 [3]湖南中医药大学第一附属医院,长沙410007 [4]郴州市第一人民医院,郴州423000

出　　处：《中华中医药杂志》2014年第9期2779-2783,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81072904;No.81273988);中国博士后科学基金项目(No.2011M501277);湖南省海外名师项目(No.2013015);湖南省中医药科研项目(No.2010007);湖南省"十二五"中医五官科学重点学科~~

摘　　要：目的:寻找不同中医证型鼻咽癌患者原发病瘤灶组织差异表达蛋白。方法:采用双向凝胶电泳对不同中医证型鼻咽癌患者的鼻咽癌组织和健康人鼻咽组织总蛋白进行分离,对比分析找出差异表达蛋白质斑点,结合基质辅助激光解吸电离-飞行时间质谱分析技术和数据库信息检索鉴定差异蛋白点。结果:与健康对照组比较,气血凝结型鼻咽癌组差异表达蛋白点共计23个,其中表达活性上调12个、表达活性下调11个,选择10个差异蛋白质斑点进行质谱鉴定,成功鉴定出5个,其中表达上调的有SH3域结合谷氨酸丰富样蛋白3、磷酸甘油酸变位酶1和烯酰水合酶辅酶1;表达下调的是α1抗胰蛋白酶和巨噬细胞帽蛋白。与健康对照组比较,火毒困结型组鼻咽癌差异表达蛋白点共计23个,其中表达活性上调12个、表达活性下调11个,选择10个差异蛋白质斑点进行质谱鉴定,成功鉴定出6个,其中表达上调的有膜联蛋白11、肌动蛋白相关蛋白3和蛋白酶体激活因子亚基2;表达下调的有谷胱甘肽S-转移酶P1、血清白蛋白和内质网蛋白29。与气血凝结型组比较,气阴两虚型组差异蛋白点共计27个,其中表达活性上调17个、表达活性下调10个,取其中10个差异点进行质谱鉴定,共有6个点得到鉴定,其中表达上调有线粒体延长因子Tu和Rab GDP解离抑制因子β;表达下调的有线粒体富马酸水合酶、免疫球蛋白KAPPA链C区、前凋亡胱冬酶衔接因子蛋白和N(G)N(G)的二甲基精氨酸二甲胺水解酶1。结论:火毒困结型、气阴两虚型和气血凝结型鼻咽癌患者原发病瘤灶组织存在差异表达蛋白质。Objective： To explore the differences of protein expression of the tumor tissues on patients with nasopharyngeal carcinoma （NPC） with different TCM patterns. Methods： Total protein of nasopharyngeal tissue of NPC patients and healthy people were separated by two-dimensional electrophoresis （2-DE）. Differential expression protein spots were compared and analyzed. Then proteins spots and the differential expression proteins were identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） combined with bioinformatics analysis. Results： Compared with healthy people, the differential expression proteins of NPC patients with syndrome of qi stagnation and blood stasis were a total of 23, with 12 express activity up-regulated and 11 express activity down-regulated. 10 different protein spots were selected to identify the mass spectrum, 5 of which were identified successfully. The expression of SH3 domain-binding glutamic acid-rich-like protein, glyceric acid phosphate mutase 1 and acryloxyethyl hydratase coenzyme 1 were increased. However,antitrypsin alpha 1 and macrophages cap protein were decreased. Compared with healthy people, the differential expression proteins of NPC patients with syndrome of heat-toxin stagnated were a total of 23, with 12 express activity up-regulated and 11 express activity down-regulated. 10 different protein spots were selected to identify the mass spectrum, 6 of which were identified successfully. The expression of membrane coupling protein 11, actin-related protein 3 and proteasome activation factor compound 2 were up-regulated, and the expression of glutathione S transferase P1, serum albumin and ERp were down-regulated. Compared with NPC patients of syndrome of qi stagnation and blood stasis, there were a total of 27 differential expression proteins, with 17 express activity up-regulated and 10 express activity down-regulated. 10 different protein spots were selected to identify the mass spectrum, 6 of which were identified s

关 键 词：鼻咽癌 中医证型 蛋白质组学 质谱分析 差异 

分 类 号：R273[医药卫生—中西医结合]