蒙药文冠木超微饮片高效液相指纹图谱研究  

Studies on HPLC fingerprint of ultramicro Xanthoceras sorbifolia Bung

作　　者：鞠爱华[1] 张静[1] 蔡丽娟[1] 庄志鹤 

机构地区：[1]内蒙古医科大学,呼和浩特010110

出　　处：《中华中医药杂志》2014年第9期2934-2937,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家"十二五"科技支撑计划(No.2012BAI28B01)~~

摘　　要：目的:采用高效液相色谱(HPLC)法对不同来源的文冠木超微饮片进行指纹图谱研究,为文冠木超微饮片质量控制提供依据。方法:色谱柱:Agilent C18(4.6mm×250mm,5μm)键合硅胶色谱柱;流动相:乙腈-0.4%磷酸(梯度洗脱);流速:1.0mL/min;检测波长:226nm;温度:20℃。结果:以16个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD%均小于2%,10批样品相似度>0.9。并指认了6个主要特征指纹峰,分别为儿茶素、表没食子儿茶素、杨梅树皮素、表儿茶素、2α,3β-双氢杨梅树皮素、双氢槲皮素。其中儿茶素为首次从文冠木药材中分离得到。结论:此方法精密度、稳定性和重复性良好,有助于文冠木超微饮片的质量控制。Objective： The fingerprint chromatograms were established for quality evaluation of ultramicro Xanthoceras sorbifolia Bung from different sources by HPLC. Methods： The analysis was performed on a Agilent C18 column （4.6mm×250mm, 5μm） with acetonitrile-0.4% phosphate acid as mobile phase in a gradient mode at a flow rate of 1.0mL/min, at the wavelength of 226nm and at a column temperature of 20℃. Results： 16 peaks were chosen as the common fingerprint peaks. The RSD for relative retention value and relative peak areas were less than 2% in the precision and repeated test. The similarity of 10 batches of samples was more than 0.9. And we identified six main characteristic fingerprint peaks including Catechin, Epigallocatechin, Epicatechin, Myricetin, 2 α, 3 β -dihydromyricetin, Dihydroquercetin. The Catechin was isolated for the first time from the Xanthoceras sorbifolia Bung medicinal materials have been isolated. Conclusion： The method is accurate, reliable and repeatable, and they can be helpful for quality control of ultramicro Xanthoceras sorbifolia Bung.

关 键 词：文冠木 超微饮片 高效液相 指纹图谱 

分 类 号：R29[医药卫生—民族医学] O657.72[医药卫生—中医学]