加味四君子汤对衰老大鼠的脾脏及细胞因子的影响  

Effects of Modified Sijunzi Decoction on spleen and cytokines of aging rats

作　　者：雷超[1] 张力华[1] 苏婷[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院,武汉430077

出　　处：《中华中医药杂志》2014年第9期2941-2944,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:观察加味四君子汤对自然衰老大鼠脾脏及其细胞因子的影响。方法:选40只12月龄健康Wistar大鼠,雌雄各半,随机分为4组,即老年组,加味四君子汤(以下简称药物)低剂量组、中剂量组、高剂量组,每组各10只;另选取10只3月龄Wistar大鼠作为青年组。老年、青年组分别给予2mL 0.9%氯化钠溶液,各给药组相应给予加味四君子汤1.6、3.2、6.4g·kg-1·d-1灌胃。持续给药90d后,称重后处死。观察大鼠的计算脾脏指数;血清免疫球蛋白(IgG)水平;将脾脏组织进行HE染色,观察组织形态学改变;并用免疫组化法检测其肿瘤坏死因子-α(TNF-α)的表达情况;以及实时荧光定量PCR方法检测核因子-κB(NF-κB)p56 mRNA的表达水平。结果:与老年组比较,高剂量组的脾脏指数、血清IgG显著降低(P<0.01),各给药组NF-κB p56显著升高(P<0.05,P<0.01),TNF-α显著降低(P<0.05);光镜下,各剂量组脾脏的组织形态较老年组有一定改善。结论:加味四君子汤可改善衰老大鼠脾脏病理形态,提高IgG和NF-κB p56水平,降低TNF-α表达并通过细胞因子之间的相互调节作用,增加机体的免疫从而延缓老龄大鼠的衰老。Objective： To observe the effects of Modified Sijunzi Decoction on spleen and cytokines of aging rats. Methods： 40 healthy Wistar rats of 12 months were randomly divided into four groups： older control group and low, medium, andhigh dose of Modified Sijunzi Decoction groups. Then 10 healthly Wistar rats of 3 months old which belonged to the youth control group were selected. The rats were sacrificed after treatment of 90 days. Observing the spleen index and serum immunoglobulin （IgG） levels. HE coloration was used to observe the morphological changes of spleen tissue. The immunohistochemical was used to test the expression of tumor necrosis factor-α （TNF-α）. The real-time quantitative PCR was used to detect the mRNA expression levels of nuclear factor-κB （NF-κB） p56 mRNA. Results： Compared with the older control group, the spleen index, serum IgG in the high dose group were increased （P〈0.01） and NF-κB p56 in drug groups increased （P〈0.05, P〈0.01）, and TNF-α decreased in drug groups （P〈0.05）. The splenic white pulp boundaries in the drug group were more clearly than that of older control group under the light microscope. Conclusion： Modified Sijunzi Decoction can improve the spleen pathological morphology of senility rats. It also can increase the levels of IgG and NF-κB p56 and reduce TNF-α expression, and increase the body＇s immune to delaying the senescence of the aged rats by regulating the interaction in cytokines.

关 键 词：衰老 四君子汤 脾脏指数 肿瘤坏死因子-α 核因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]