茄子高频再生体系的建立与优化  

Establishment and Optimization of High-frequency Regeneration System of Eggplant

作　　者：李烨[1] 金丹丹[2] 谢立波[3] 李景富[2] 

机构地区：[1]哈尔滨市农业科学院,黑龙江哈尔滨150001 [2]东北农业大学,黑龙江哈尔滨150030 [3]黑龙江省农业科学院,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2014年第9期48-52,共5页Heilongjiang Agricultural Sciences

摘　　要：为建立并优化茄子高频再生体系,研究以茄子品种哈农杂茄2号和哈农杂茄3号为材料,利用不同的外植体和植物生长调节剂组合,探讨影响茄子再生频率的主要因素。结果表明:茄子最佳再生子叶最适分化培养基为MS 0+6-BA 1mg·L-1+KT 2mg·L-1;下胚轴最适分化培养基为MS 0+KT 2mg·L-1,再生苗最佳生根培养基为1/2MS+IAA 0.1mg·L-1,且下胚轴的分化效率高于子叶。In order to establish and optimize the high-frequency regeneration system of eggplant ,taking cotyle-donary petiole and hypocotyl explants of Hanongzaqie 2 and Hanongzaqie 3 as materials ,the shoot regeneration frequencies of two eggplant（Solanum melongena L）cultivars were examined to discuss the factors affecting re-generation rate .The results showed that the optimum shoot inducing mediums for cotyledons was MS 0＋6-BA 1 mg·L-1 ＋KT 2 mg·L-1 ;the optimum shoot inducing mediums for hypocotyls was MS 0＋ KT 2 mg·L-1 ,the best rooting medium for regeneration seedling was 1/2MS＋IAA 0 .1 mg·L-1 .And the differentiation of hypo-cotyls was higher than that of cotyledons .

关 键 词：茄子 外植体 再生 

分 类 号：S641.1[农业科学—蔬菜学]