大豆根际解磷细菌的分离及筛选  

Isolation and Screening of Phosphate-solubilizing in the Rhizosphere of Soybean

作　　者：李丽丽[1,2,3] 郎敬[4] 杨洪一[4] 邰飞飞 来永才[1] 

机构地区：[1]黑龙江省农业科学院耕作栽培研究所,哈尔滨150086 [2]东北林业大学博士后科研流动站/黑龙江省农业科学院博士后科研工作站,哈尔滨150040 [3]黑龙江省林业科学研究所,哈尔滨150081 [4]东北林业大学生命科学学院,哈尔滨150040

出　　处：《湖北农业科学》2014年第13期3048-3050,共3页Hubei Agricultural Sciences

基　　金：黑龙江省自然科学基金项目(QC2011C110);哈尔滨市科技创新人才基金项目(2012RFQYN023);中国博士后科学基金项目(2011M500706;2012T50382);黑龙江省博士后科学基金项目(LBH-Z11031);黑龙江省农业科学院博士后基金项目

摘　　要：解磷菌对于提高土壤中可溶性磷的含量、促进植物对磷的吸收具有重要作用。利用PVK培养基对黑龙江省海伦、北安地区大豆根际土壤中的解磷细菌进行初筛,共分离得到8株具有解无机磷能力的菌株。利用NBRIP培养基进行摇瓶复筛,培养3 d后,根据摇瓶中上清液的可溶性磷量的高低,筛选出5株解磷能力较强的菌株。其中海伦Ⅳ号的解无机磷能力最强,其培养液上清中的溶磷量可提高约10倍。研究中的技术体系可有效获得溶磷的细菌菌株,为进一步开发细菌磷肥打下了基础。The phosphate-solubilizing bacteria in the soil play an important role for improving the phosphorus level in the soil and absorbing the phosphorus nutrition for plants.The phosphate-solubilizing bacteria （PSB） in the rhizosphere of soybean was isolated using modified PVK medium.Eight strains of PSB were isolated.Subsequently,5 strains of high-effective PSB were isolated according to phosphorus level in supernatant of culture solution after 3-day incubation in a liquid medium NBRIP.Hailun Ⅳ was found to possess the highest phosphate-solubilizing ability among 5 strains under the screening conditions.The soluble P in the supernatant of the culture of Hailun Ⅳ increased about 10-fold.The screening system can effieiently obtain PSB and is helpful to develop PSB phosphorus fertilizer.

关 键 词：解磷细菌 溶磷量测定 大豆 

分 类 号：S154.3[农业科学—土壤学]