非平衡法在乙型肝炎e抗体和核心抗体检测中的效果评价  

Evaluation of Non Equilibrium Method in the Detection of e Antibody and Core Antibody in Hepatitis B

作　　者：郭辉 

机构地区：[1]上海计生所医院检验科,上海200032

出　　处：《标记免疫分析与临床》2014年第4期453-454,共2页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨酶联免疫吸附试验非平衡法对乙型肝炎e抗体和核心抗体检测结果的影响。方法收集电化学发光免疫分析法(ECLIA)检测抗-HBe阳性标本44例和抗-HBc阳性标本57例,再用酶联免疫吸附试验法(ELISA)对阳性标本进行检测,样本加入反应孔中分别于0min、30min后加入酶标志物,余下步骤均严格按照说明书操作。结果对ECLIA检测抗-HBe阳性标本44例、抗-HBc阳性标本57例进行ELISA复检后,平衡法抗-HBe的漏检率为52.3%,抗-HBc漏检率为40.4%;非平衡法抗-HBe的漏检率为34.1%,抗-HBc漏检率为24.6%。结论竞争法检测抗-HBe和抗-HBc时应适当延迟加酶的时间,以降低漏检率。Objective To explore the influence of enzyme- linked immunosorbent assay method of non- equilibrium on the detection of e antibody and core antibody in hepatitis B. Methods 44 cases of anti-HBe positive and 57 eases of anti-HBc positive specimens determined by electrochemical luminescence immunoassay were analyzed by enzyme linked immunosorbent again. The enzyme marker were added into reaction holes at 0 min, 30 min after samples add respectively, the remaining steps were followed in strict accordance with the manual operation. Results The miss positive detection rate of anti-HBe and anti-HBc analyzed by equilibrium method were 52.3% and 40.4% respectively. The miss positive detection rate of anti-HBe and anti-HBc analyzed by non equilibrium method were 34.1% and 24.6% respectively. Conclusion It would be better to prolong the adding time of enzyme in the detection of anti-HBe and anti-HBc by enzyme-linked immunosorbent assay in order to increase the detection sensitivity.

关 键 词：酶联免疫吸附试验 乙型肝炎病毒e抗体 乙型肝炎病毒核心抗体 

分 类 号：R446.6[医药卫生—诊断学]