菌种类型及EDTA二钠用量对生物甲烷生成的影响  

Effects of Strains Type & the Amount of Bi-Sodium EDTA on Bio-Methane Production

作　　者：夏大平[1,2] 司青[1] 苏现波[1,2] 胥帅帅 马俊强[1] 

机构地区：[1]河南理工大学,河南焦作454003 [2]瓦斯地质与瓦斯治理国家重点实验室培育基地,河南焦作454003

出　　处：《微生物学杂志》2014年第4期15-20,共6页Journal of Microbiology

基　　金：国家自然科学基金(41002047);河南理工大学校内青年基金(Q2012-31);省部共建国家重点实验室培育基地开放基金项目(WS2013A12);河南省教育厅科技攻关项目(13A440335)

摘　　要：采用平板分离和培养瓶厌氧技术,结合PCR扩增技术,以沙曲矿煤为发酵基底,中泰矿业和沙曲矿井水及白腐菌为菌种来源,进行产甲烷菌培养实验,并添加不同浓度梯度的EDTA二钠,进行产甲烷菌培养实验,测定实验结束后的产甲烷量。实验证明:中泰矿业矿井水中有产甲烷菌的存在,而沙曲矿井水在含反硝化菌和pH偏高的情况下,抑制了产甲烷菌的生长。同等条件下沙曲矿批次实验中没有甲烷气体的产生,而中泰矿业的有气体产出,适宜浓度的EDTA二钠浓度会显著提高产气量,EDTA二钠用量为1.0 g/L时,中泰矿井水CH4产率达到最大,为10.0 mL/g煤,反应后溶液的pH值明显增高。Adopting the plate separation technology and incubation bottles anaerobic technology,combined with the PCR amplification technology,using coal samples from Shaqu coal mine as the fermentation substrates and the mine water respectively from Zhongtai Mining and Shaqu mine and white rot fungi as the strain sources,the methane-produ-cing bacteria cultivation experiment was conducted and the methane production was determined by adding the bi-sodi-um EDTA solution with various concentration gradients. The experimental had confirmed that there exist methane-pro-ducing bacteria in the mine water of Zhongtai Mining,and under the conditions of the content of denitro-bacteria and slantly high pH in the mine water of Shaqu coal mine the growth of methane-producing bacteria was inhibited. Under equal conditions,there is no methane produced in the batch experiments coal samples from Shaqu mine,while there is a gas output in the samples of Zhongtai Mining,and appropriate concentration of bi-sodium EDTA significantly in-creased the gas production. When the bi-sodium EDTA was at 1. 0 g/ L,the CH4 production rate reached the maxi-mum at 10. 0 mL/ g,and the pH value increases notably after the reaction.

关 键 词：产甲烷菌 生物甲烷 EDTA 二钠 产气量 

分 类 号：Q939.97[生物学—微生物学]