EBNA3C与Gemin3两种蛋白形成复合物及相互作用的结构域  

Compound Formed by Two Proteins EBNA3C & Gemin3 and Their Interaction Structural Domain

作　　者：郭毅[1] 唐艳君 赵楠[1] 

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001 [2]鞍山市岫岩满族自治县第三人民医院妇产科,辽宁鞍山114300

出　　处：《微生物学杂志》2014年第4期27-32,共6页Journal of Microbiology

基　　金：国家自然科学基金(81171649);辽宁省自然科学基金(201102267)

摘　　要：探讨EB病毒核抗原3C(EBNA3C)与Gemin3的相互结合及其作用区域。用Flag-EBAN3C与GFPGemin3共转染HEK 293细胞,采用免疫共沉淀、谷胱苷肽-S转移酶共沉淀及免疫荧光蛋白共存实验确定EBNA3C与Gemin3两种蛋白在体内、体外相互结合及相互作用的结构域。EBNA3C与Gemin3两种蛋白在体内外相结合,二者通过各自的C端相互结合。EBNA3C与Gemin3两种蛋白形成稳定复合物。Interaction and working domain of nucleo-antigen 3C of EB virus（EBNA3C）with Gemin3 was investiga-ted. HEK 293 cells were co-infected with Flag-EBNA3C and GFP-Gemin3,and their structural domain of two proteins EBNA3C and Gemin3 inter-conjunction and interaction in vivo and in vitro was confirmed by co-existence experiment adopting immune co-precipitation,glutathione-S transferase and immunofluorescence protein. EBNA3C and Gemin3 combined with each other in vivo and in vitro through their individual C-terminal. Two kinds of proteins of EBNA3C and Gemin3 formed a stable compound.

关 键 词：EBNA3C Gemin3 结构域 

分 类 号：Q939.96[生物学—微生物学] R466.5[医药卫生—临床医学]