脐血粒系祖细胞发育过程中HoxB8的表达及干预  

In vitro intervention study of HoxB8 expression in the developing process of granulocyte progenitor

作　　者：江南静[1] 刘文君[1] 

机构地区：[1]泸州医学院附属医院儿科,四川泸州646000

出　　处：《泸州医学院学报》2014年第4期384-389,共6页Journal of Luzhou Medical College

基　　金：四川省卫生厅资助项目(编号20060040)

摘　　要：目的:探讨人脐血造血干细胞(HSC)向粒系祖细胞(CFU-G)发育过程中,全反式维A酸(ATRA)和(或)三氧化二砷(As2O3)对HoxB8 mRNA/蛋白表达的影响,为ATRA和As2O3治疗提供依据。方法:12例脐带血样本采自胎盘段脐带血,在人脐血造血干细胞(HSC)向粒系祖细胞(CFU-G)增殖分化过程中,加入ATRA 10 nmol/L和(或)As2O310 nmol/L持续干扰培养的细胞。采用real-time PCR和Western Blotting方法分别测定HoxB8 mRNA/蛋白的表达水平。结果:人类造血干细胞向粒系祖细胞增殖分化过程中,对照组、ATRA组、As2O3组及ATRA+As2O3组的HoxB8 mRNA/蛋白第3 d开始表达,第7 d表达较强烈,第12 d表达减弱;在real-time PCR方法中,与对照组比较,ATRA组、As2O3组及ATRA+As2O3组HoxB8 mRNA的表达上调(P<0.05)。在Western Blotting方法中,与对照组比较,ATRA组、As2O3组及ATRA+As2O3组HoxB8蛋白的表达上调(P<0.05)。结论:在人脐血造血干细胞(HSC)向粒系祖细胞(CFU-G)发育过程中,HoxB8基因与粒系造血密切相关。ATRA、As2O3及ATRA+As2O3能显著上调HoxB8 mRNA及蛋白的表达。Objective： To investigate the effect of all-trans retinoic acid（ATRA）and arsenic trioxide（As2O3）on homeoboxB8（HoxB8）mRNA and protein expressions. Methods： Twelve cord blood samples were collected from the fetal placenta umbilical vein and cultured in vitro. The proliferation and differentiation of cord blood HSCs into CFU-G was continuously disrupted with 10 nmol/L of ATRA and/or 10 nmol/L of As2O3. The expression of HoxB8 mRNA and protein were detected by quantitative real-time polymerase chain reaction（qRT-PCR） and Western Blotting, respectively. Results： HoxB8 mRNA/protein expression was detectable on the 3rd day, reached its highest level on the 7th day and decreased on the 12 th day. HoxB8 mRNA/protein expression in ATRA,As2O3,and ATRA＋As2O3groups was upregulated compared with those in control group（P 〈0.05）. Conclusion：There is positive relationship between HoxB8 gene and granulocyte progenitor hematopoiesis. ATRA/As2O3up-regulate the expression of HoxB8 mRNA/protein, and treatment of leukemia with ATRA/As2O3 may be through the regulation of Hox gene expression.

关 键 词：粒系祖细胞 全反式维甲酸 三氧化二砷 HoxB8基因 

分 类 号：R331.142[医药卫生—人体生理学]