甲磺酸伊马替尼增加顺铂对耐药卵巢癌细胞作用的研究  

Study on imatinib mesylate increasing the effect of cisplatin on cisplatin-resistant ovarian tumor cell

作　　者：张阔[1] 陈燕[1] 

机构地区：[1]河北联合大学附属医院妇科,河北唐山063000

出　　处：《实用药物与临床》2014年第9期1096-1099,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的探讨靶向治疗药物甲磺酸伊马替尼(Imatinib mesylate)对耐顺铂人卵巢腺癌细胞株SKOV3/DDP的增殖抑制和诱导凋亡作用以及与顺铂合用的效果。方法 MTT法检测不同浓度甲磺酸伊马替尼、DDP以及联合用药对人卵巢癌耐顺铂细胞株SKOV3/DDP的增殖抑制情况,采用双染色流式细胞术检测甲磺酸伊马替尼对SKOV3/DDP的诱导凋亡作用。结果 MTT法检测出不同药物浓度的甲磺酸伊马替尼作用于SKOV3/DDP细胞呈现细胞生长抑制作用,甲磺酸伊马替尼与不同浓度顺铂联合用药对细胞生长抑制率分别为38.63%、51.55%、66.54%,明显高于DDP单用药组的8.30%、16.68%、30.83%,两组比较差异有统计学意义(P<0.05)。两药合用后的IC50为9.57,是单用顺铂组的4.13倍。甲磺酸伊马替尼与顺铂合用后,早期凋亡率由1.25%增加到12.31%(P<0.05)。结论甲磺酸伊马替尼可以抑制卵巢癌耐DDP细胞株SKOV3/DDP的增殖和诱导细胞凋亡,并显著增强其对DDP的敏感性。Objective To study the effects of imatinib mesylate on proliferation,apoptosis and cell resistance of drug-resistant ovarian cancer cell line SKOV3/DDP.Methods MTT assay was used to examine the inhibition effect of different concentrations of imatinib mesylate,DDP and combination drugs use on proliferation of ovarian cancer cell line SKOV3/DDP,and flow cytometry was used to detect the apoptosis of SKOV3/DDP induced by imatinib mesylate.Results MTT showed that different concentrations of imatinib mesylate in SKOV3/DDP cells had inhibition effect.The inhibition rates of imatinib mesylate and cisplatin combination in different concentrations were 38.63％,51.55 ％,66.54 ％,which were significantly higher than those for DDP （8.30％,16.68 ％,30.83 ％）,there were signifi cant differences between the two groups（P ＜ 0.05）.IC50 of combination was 9.57,which was 4.13 times for that of cisplatin alone.When cisplatin was used combination with imatinib mesylate,early apoptosis rate increased from 1.25 ％to 12.31％ （P ＜ 0.05）.Conclusion Imatinib mesylate can inhibit DDP resistant ovarian cancer cell lines SKOV3/ DDP proliferation and induce cell apoptosis,and significantly enhance its sensitivity to the DDP.

关 键 词：甲磺酸伊马替尼 顺铂 卵巢癌 耐药 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]