利用pHsh载体克隆与表达双活性阿拉伯/木糖苷酶  

Cloning and Expression of a Arabinosidase-xylosidase from Thermoanaerobacter ethanolicus JW200 Using pHsh Vector

作　　者：彭静静[1] 

机构地区：[1]泰山学院生物与酿酒工程学院,山东泰安271021

出　　处：《湖北农业科学》2014年第15期3662-3664,共3页Hubei Agricultural Sciences

基　　金：泰安市科技发展计划项目(20132094);泰山学院博士科研启动金项目(Y-01-2013001)

摘　　要：将来源于嗜热厌氧乙醇菌(Thermoanaerobacter ethanolicus JW200)的双活性阿拉伯/木糖苷酶(XarB)构建到新型热激质粒pHsh上,得到重组质粒。将重组质粒pHsh-xarB转入大肠杆菌(Escherichia coli JM109)。SDS-PAGE结果表明,该重组酶的分子量为86 kDa,与理论值相符。基于热激载体pHsh的重组表达系统具有诱导表达简便、诱导方式廉价的优点,且重组酶热稳定性好。The structure gene from Thermoanaerobacter ethanolicus JW200 encoding XarB gene was amplified and ligated into pHsh vector, resulting in pHsh-xarB. XarB gene was obtained after expressing pHsh-xarB in Escherichia coli JM109. Results of SDS-PAGE showed that the molecular weight of the recombinant XarB expressed was about 86 kDa, the same as the size predicted. The expression vector system of the heat shock plasmid pHsh had high expression level and cheap induction.

关 键 词：阿拉伯/木糖苷酶 pHsh 克隆 表达 

分 类 号：Q814[生物学—生物工程]