利用表型数据构建狗牙根初级核心种质  

Constructing pre-core collection of Cynodon dactylon based on phenotypic data

作　　者：郑轶琦[1,2] 郭琰[1] 房淑娟[1] 徐亚楠[1] 陈静波 刘建秀 

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]江苏省中科院植物所,江苏南京210014

出　　处：《草业学报》2014年第4期49-60,共12页Acta Prataculturae Sinica

基　　金：国家自然科学基金(31101754;31101567)资助

摘　　要：本研究以831份狗牙根种质资源为试材,根据15个数量和质量性状,研究了狗牙根初级核心种质构建的方法。从总体取样规模(5%,10%,15%,20%,25%和30%)、组内取样比例(简单比例、对数比例、平方根比例和多样性比例)和取样方法(随机取样和聚类取样)3个层次探讨构建狗牙根初级核心种质的最佳取样策略,共形成48种取样策略。应用均值差异百分率、方差差异百分率、极差符合率和变异系数变化率4个参数来检验各取样策略的优劣。比较初始种质和初级核心种质两者基于主成分的样品分布图及性状间的相关系数,对构建的初级核心种质进行确认。结果表明,25%是最适宜的总体取样比例;4种组内取样比例中平方根比例优于简单比例、对数比例和多样性比例;2种取样方法中,聚类取样明显优于随机取样。最终确定25%的总体取样规模下,组内采用平方根比例和聚类抽样方法是构建狗牙根初级核心种质的最佳取样策略。根据获得的最佳取样策略最终在表型水平建立了包含208份种质的狗牙根初级核心种质,该种质可以作为整体资源的代表性样本,在狗牙根种质资源的充分开发和利用中发挥重要作用。In this study,the method of constructing Cynodon dactylon pre-core collection was studied based on15 quantitative and qualitative traits from 831 accessions.An optimal sampling scheme was selected from 48 strategies which consisted of sampling proportions（5%,10%,15%,20%,25%,and 30%）,sampling proportions within group（simple proportion,logarithmic proportion,square root proportion and genetic proportion）and individual selection methods within group（random selection and cluster selection）evaluating with four effective parameters including mean difference percentage,variance difference percentage,coincidence rate of range and changeable rate of variation coefficient.The optimal sampling scheme was also confirmed by comparing the accessions distribution plotted by first two principal components,correlation coefficients of traits between two collections.The result indicated that 25% was the optimum sampling proportions.The square root proportion was better than simple proportion,logarithmic proportion and genetic proportion.The cluster selection was more suitable than random selection.At 25%sampling proportion,using square root proportion within group and cluster selection method within group is the most suitable method for constructing C.dactylon pre-core collection.The result conducted by PCA analysis showed the genetic structure and variation of 831 accessions was well represented by the pre-core collection.The most important phenotypic correlations between traits which may be under the control of co-adapted gene complexes were also preserved by the pre-core collection.Based on the optimized sampling strategy,the pre-core collection consisting 208 accessions of C.dactylon were established and will play an important role in the utilization of C.dactylongenetic resources.

关 键 词：狗牙根 表型数据 初级核心种质 取样策略 

分 类 号：S688.4[农业科学—观赏园艺]