外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响  

Effects of exogenous betaine on the secondary metabolites of Pinellia ternata under drought stress

作　　者：张红敏[1] 李姣姣[1] 黑刚刚[1] 曹瑞霞[1] 周心渝[1] 李琳琳[1] 杨卫星[1] 吴能表[1] 

机构地区：[1]西南大学生命科学学院三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《草业学报》2014年第4期229-236,共8页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(30500041);重庆市科技攻关计划项目(cstc2012gg-yyjs0619)资助

摘　　要：为探讨喷施外源甜菜碱溶液对干旱胁迫下半夏体内相关次生代谢产物及氮代谢相关酶活性的影响,采用盆栽控水法研究了外源甜菜碱对D（干旱处理,含水量35%±2%）、D20（干旱处理并加喷20mmol/L的甜菜碱）、D40（干旱处理并加喷40mmol/L的甜菜碱）各处理组半夏体内谷氨酸合酶（GOGAT）活性、谷氨酰胺合成酶（GS）活性、谷氨酸脱氢酶（GDH）活性、硝态氮、可溶性蛋白、总生物碱、鸟苷和腺苷含量的变化规律。结果表明随着干旱胁迫时间的增加,D40中GOGAT活性及GS活性降低幅度均小于D、D20,GDH活性呈先升高后降低的趋势,对照组（CK组,含水量75%±2%）变化不明显。在处理第9-27天时,D40与D20相比,总生物碱含量分别提高了6.3%,10.3%,12.3%,差异显著。腺苷和鸟苷含量随时间增加呈上升趋势,且D40含量均高于D20,CK组含量最高,腺苷在第27天时有降低趋势。在第27天时,D40可溶性蛋白含量分别比D、D20提高了7.3%和3.1%。表明40mmol/L的外源甜菜碱溶液能提高干旱胁迫条件下氮代谢相关酶的活性,从而加快无机氮的同化,有利于促进次生代谢物质的积累,同时也增强了半夏抵御干旱胁迫的能力。To explore the effects of exogenous betaine solution on secondary metabolites and nitrogen metabolism enzyme activities of Pinellia ternatain a drought environment,a water pot control experiment was used.The treatments were D（drought treatment,35%±2% moisture content）,D20（drought treatment with spray of 20mmol/L betaine）,and D40（drought treatment with spray of 40mmol/L betaine）.Glutamate synthase（GOGAT）,glutamine synthetase（GS）,glutamic dehydrogenation enzyme（GDH）,nitrate,soluble protein,alkaloids,guanosine and adenosine contents of P.ternata were measured in each treatment group.As drought time increased,the GOGAT and GS activities of D40 decreased slower than those of D,and D20,while GDH activity initially increased but then decreased.However,the CK（control group,75%±2% moisture content）group did not change significantly.The alkaloids in the D40 group improved 6.3%（9days）,10.3%（18days）and 12.3%（27days）,which was higher than those in the D20.The nitrate,alkaloids and guanosine contents increased with time were higher in the D40 group than in the D20 group,although the CK group was the highest.The adenosine content tended to decrease when the drought stress time reached 27 d.At 27 d,the content of soluble protein in the D40 group was 7.3%higher than in the D,as well as 3.1%higher than in the D20.The 40mmol/L exogenous betaine solution concentrations increased nitrogen metabolism enzyme activities in P.ternataunder drought conditions.This may accelerate the inorganic nitrogen assimilation,which is conducive to the accumulation of secondary metabolites and enhances the ability of P.ternatato resist drought stress.

关 键 词：半夏 干旱胁迫 甜菜碱 氮代谢 

分 类 号：S567.239[农业科学—中草药栽培] Q945.78[农业科学—作物学]