新疆猪繁殖与呼吸综合征病毒分离鉴定及其Nsp2和ORF5基因遗传变异分析  

Identification and sequence analysis of Nsp2 gene and ORF5 gene of porcine reproductive and respiratory syndrome virus isolated from Xinjiang

作　　者：张美玲[1,2] 陆桂丽[1] 薛晶[1] 刘丽雅[1] 简子健[2] 夏俊[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《广东农业科学》2014年第17期106-110,2,共5页Guangdong Agricultural Sciences

基　　金：新疆维吾尔自治区科研院所基本科研业务经费项目

摘　　要：为探索新疆猪繁殖与呼吸综合征病毒（PRRSV）的变异特征,利用RT-PCR、ORF5和部分NSP2基因序列进行分析,鉴定从新疆某猪场疑似猪高热病死亡猪肺脏分离出的一株病毒.分离的病毒株被鉴定为PRRSV美洲型,命名为XJ-Q株.该株病毒与香港分离株HK13亲缘关系最近,与国内变异株（HUB1和JXA1）同源性较为亲近.与美洲型标准毒株VR-2332相比,该分离株在481位和532～560位共有30个氨基酸缺失;与国内分离的PRRSV变异株相比,在487～489位有3个氨基酸的独特缺失.结果表明,新疆分离株为PRRSV美洲型,属于新缺失的PRRSV毒株.The variant characteristics of the porcine reproductive and respiratory syndrome virus （PRRSV） isolated from Xinjiang were studied. Identification of the PRRSV strain isolated from lungs in the clinical diseased piglets was performed by RT-PCR and sequence analysis of Nsp2 gene and ORF5 gene. The sequence analysis showed that the Xinjiang isolate belonged to the American strain of PRRSV, named XJ-Q. It was very closer to Hongkong HK13 and homology of XJ-Q was 93.6%-99.8%, next to HUB1 and JXA1 isolates in China with 92.4% homology. However, the Nsp2 protein of XJ-Q had a total of 30 amino acids deletion between aa532 and aa560, compared with that of North American standard strain VR-2332 in Gene Bank, and also had another unique 3 amino acids deletion between aa487 and aa489 within its Nsp2-coding region, compared with those of other variant PRRSV strains isolated from China in recent years. The analysis showed that XJ-Q isolate represents a newly emerged PRRSV variant, belonging to the American strain of PRRSV.

关 键 词：猪繁殖与呼吸道综合征病毒(PRRS) ORF5基因 NSP2基因 序列分析 

分 类 号：S852.651[农业科学—基础兽医学] Q754[农业科学—兽医学]