毛白杨AP2/ERF类转录因子PtoSHN的克隆及分析  

Cloning and analysis of AP2/ERF transcription factor PtoSHN in Populus tomentosa

作　　者：曹凌燕[1,2] 陈亚娟[2] 王宏芝[2] 张杰伟[2] 丁莉萍[2] 魏建华[2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农业生物技术研究中心,北京100097

出　　处：《广东农业科学》2014年第17期140-144,共5页Guangdong Agricultural Sciences

基　　金：国家"863"计划项目(2013AA102702)

摘　　要：AP2/ERFs是一个庞大的转录因子家族,在植物的生长发育过程中发挥着重要作用。以毛白杨(Populus tomentosa)为材料,克隆了毛白杨AP2/ERF类转录因子PtoSHN的编码区序列。组织表达特异性结果显示,PtoSHN基因在毛白杨茎中高水平表达。系统进化分析表明,PtoSHN与拟南芥AtSHN2和AtSHN3相似度较高,其中AtSHN2已被证明参与了植物次生壁的合成调控。通过亚细胞定位预测,PtoSHN定位于细胞核内。亚细胞定位结果表明,PtoSHN定位在核内。表明已成功克隆到毛白杨SHN基因,该基因可能在林木次生生长过程中起调控作用。AP2/ERF is a large family of transcription factors in plants, which has great effect on plant growth and physiology. The full-length coding sequence of transcription factor SHINE （SHN） from Populus tomentosa was cloned. Real-time PCR results showed that PtoSHN expressed in various organs in poplar, especially in the stem. Phylogenetic tree proved that PtoSHN shared a high similarity with AtSHN2 and AtSHN3 from Arabidopsis thaliana. The AtSHN2 has proved to be involved in plant secondary wall synthesis regulation. PtoSHN located in the nucleus, which was predicted by bioinformational software CELLO v.2.5, and then testified by subcellular localization. Therefore, we successfully cloned SHN from P. tornentosa and speculated that SHN gene may play a key role in regulating trees＇ secondary growth.

关 键 词：毛白杨 SHN 次生生长 亚细胞定位 

分 类 号：S718.466[农业科学—林学]