农杆菌介导的华南型黄瓜遗传转化体系的初步研究  

Establishment of Genetic Transformation System by Agrobacterium tumefaciens of Cucumber

作　　者：杨宏[1,2] 刘小俊[1,2] 梁根云[1,2] 房超[1,2] 刘独臣[1,2] 蔡鹏[1,2] 李跃建[1,2] 

机构地区：[1]四川省农业科学院园艺研究所蔬菜种质与品种创新四川省重点实验室,四川成都610066 [2]农业部西南地区园艺作物生物学与种质创制重点实验室,四川成都610066

出　　处：《西南农业学报》2014年第4期1656-1660,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省农业科学院青年基金(2011QNJJ-011);国家现代农业产业技术体系专项资金(CARS-25-G-43);国家国际科技合作专项资金(KY-2012-15-05)

摘　　要：以华南型黄瓜‘二早子’、‘白丝条’、‘川绿2号’近叶柄部子叶为外植体,对影响农杆菌介导的卡那霉素抗性遗传转化体系的主要因素如基因型、不定芽诱导最佳激素配比、筛选压、农杆菌侵染时间、农杆菌侵染浓度、共培养温度等进行试验,建立一个高效的黄瓜子叶遗传转化体系。结果表明,子叶分化再生能力‘川绿2号>‘二早子’>‘白丝条’。1.5 mg/L 6-BA+0.1mg/L TDZ+0.5 mg/L ABA能较好地促进‘二早子、‘白丝条子叶不定芽再生,而"川绿2号"在含1.5 mg/L 6-BA+0.5 mg/LABA的MS培养基就能达到最佳分化率。筛选压与外植体分化能力有关,分化能力强的基因型需要较高的kan筛选压。OD600=0.3,侵染60min,共培养温度26℃时有利于子叶的遗传转化。本研究中,‘二早子’的遗传转化率达到15.46%,‘白丝条’的遗传转化率达到10.2%。A transformation system of cucumber cotyledon via Agrobacterium tumefaciens were established by studying factors including geno- type, inducing medium with different hormones, screening pressure, GV3101 concentration, infection time, co-cultivation temperature, etc. The results indicated that, MS ＋ 1.5 mg/I.6-BA ＋ 0. lmg/L TDZ ＋0.5 mg,/L ABA were good for adventitious bud regeneration of ＇ erzaozi ＇ and＇ baisitiao＇. Cotyledon differentiation ability ＇ cbuanlv 2 ＇ 〉 ＇ erzaozi＇ 〉 ＇ balsitiao＇ , genotype of high differentiation ability required high Kan screening pressure. GV3101 concentration of OD^o 0.3, 60 min for infection time, and co-cultivation temperature with 26 ℃ were the best transformation condition. The transformation frequency of ＇ erzaozi＇ and＇ baisitiao＇ were 15.46 % and 10.2 %, repectively.

关 键 词：华南型黄瓜 农杆菌 遗传转化 因素 

分 类 号：S642.2[农业科学—蔬菜学]