川贝母悬浮培养细胞活力测定条件的优化  

Determination Condition Optimization of Suspension Culture of Fritillaria Cell

作　　者：王跃华[1] 何诗虹 郭翠平[1] 江明殊[1,2] 王晓蓉 刘涛[1] 

机构地区：[1]成都大学生物产业学院,四川成都610016 [2]四川师范大学生命科学院,四川成都610101 [3]成都恩威集团(投资)有限公司,四川成都610041

出　　处：《西南农业学报》2014年第4期1691-1694,共4页Southwest China Journal of Agricultural Sciences

基　　金：成都市科技计划项目(12GGYB413SW-001);成都市经济和信息化委员会资助的科研项目(2012)

摘　　要：为了利用四氮唑脱氢酶还原法测定川贝母悬浮培养细胞的活力,并对影响其活力测定的各种因素进行了研究.选取四氮唑脱氢酶还原法测定了经三次继代培养的川贝母悬浮培养细胞的活力,并研究了不同四氮唑溶液浓度、缓冲液种类和pH值以及脱氢酶还原反应时间对川贝母悬浮培养细胞活力测定的影响.通过实验得出用四氮唑脱氢酶还原法测定川贝母悬浮细胞活力的最佳条件为：四氮唑溶液最适浓度0.6％,脱氢酶还原反应在Tris-HCl缓冲液中的效果优于磷酸缓冲液和磷酸氢二钠-柠檬酸缓冲液,最适pH为7.0 ～7.5;脱氢酶最适还原反应的时间为18 h.The viability of frltillaria cell by suspension culture was measured by TrC dehydrogenase reduction method to study the effects of different tetrazole concentration,buffer typos,pH and the the reduction reaction time on the cell viability. The sults showed that the optimum conditions of the viability determination of fritillaria cell were that the optimum tetrazole concentration was 6 % ,the Tris-HC1 buffer was supe- rior to the phosphate buffer and disodium hydrogen phosphate-citrlc acid buffer,the optimum pH was 7.5 and the optimum reductlonreaction time was 18 h.

关 键 词：氯化三苯基四氮唑 脱氢酶 细胞活力 

分 类 号：S567.231[农业科学—中草药栽培]