胃痞消对胃癌前病变大鼠胃黏膜组织病理学的影响  

Influence of Weipixiao on Histopathological Changes of Gastric Mucosal Tissue in Rats with Gastric Precancerous Lesions

作　　者：曾进浩[1] 潘华峰[1] 刘友章[1] 严艳[1] 赵自明[2] 任金玲[1] 李海文[1] 胡霞[1] 赖秋华 

机构地区：[1]广州中医药大学,广东广州510006 [2]广东省中医研究所,广东广州510095

出　　处：《广州中医药大学学报》2014年第5期768-771,846,847,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81273739);广东省自然科学基金资助项目(编号:S2011010003685);广东省研究生教育创新计划项目(编号:2013JDXM20)

摘　　要：【目的】观察健脾化瘀解毒复方胃痞消对胃癌前病变(gastric precancerous lesions,GPL)大鼠胃黏膜组织病理学的影响。【方法】将SD大鼠随机分为正常组,模型组,维酶素组(剂量为0.2 g·kg-1·d-1),胃痞消高、中、低剂量组(剂量分别为15、7.5、3.75 g·kg-1·d-1)。采用N-甲基-N’-硝基-N-亚硝基胍(MNNG)饮用液自由饮用、饥饱失常、耗气泻下法复制GPL大鼠模型,连续18周。从第9周起,给药组按上述剂量连续灌胃给药共10周,观察胃痞消治疗后大鼠胃黏膜上皮的病理学改变。【结果】模型组大鼠胃黏膜肠上皮化生及异型增生的病理积分均较正常组显著升高(P<0.01);胃痞消各剂量组病理学改变均有不同程度改善,并以胃痞消低剂量组作用为佳(P<0.05或P<0.01)。【结论】胃痞消可以从一定程度上阻断和逆转GPL大鼠胃黏膜肠上皮化生及异型增生病变,低剂量胃痞消防治胃癌前病变的远期疗效更佳。Objective To observe the effect of Weipixiao, a compound recipe which has the actions of strengthening spleen, resolving stasis and removing toxins, on the histopathological changes of gastric mucosal tissue in rats with gastric precancerous lesions （GPL） . Methods SD rats were randomly divided into normal group, model group, Vitacoenzyme group （0.2 g·kg-1·d-1）, and high-, middle-, and low-dose Weipixiao groups （in the dose of 15, 7.5, 3.75 g·kg-1·d-1, respectively） . Except for the normal control group, the rats in other groups received spontaneous intake of N-methyl-N＇-nitro-nitrosoguanidine （MNNG） solution combined with irregular diet and oral use of purgative herbs for 18 weeks to induce GPL. From the 9th week, the mediation groups were simultaneously given corresponding medicine for 10 weeks. At the end of the experiment, the histopathological changes of gastric mucosal tissue in all groups were observed. Results Pathological scores of intestinal metaplasia and epithelial dysplasia in rat gastric mucosa of the model group were significantly increased （P〈0.01 compared with those of the normal group）, but were decreased in three Weipixiao groups to various degrees, particularly in low-dose Weipixiao group （P〈0.05 or P〈0.01） . Conclusion Weipixiao can block and reverse gastric intestinal metaplasia and dysplasia in GPL rats to certain degrees, and low-dose Weipixiao may have better long-term effect for the prevention and treatment of GPL.

关 键 词：胃痞消/药理学 胃癌前病变/中药疗法 胃黏膜/病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]