清咽合剂利咽、抗炎和止咳作用研究  

Experimental Study of Sore-throat-relieving, Anti-inflammation and Antitussive Action of Qingyan Mixture

作　　者：郑志文 朱碧媛 

机构地区：[1]广东省东莞市中医院,广东东莞523000

出　　处：《广州中医药大学学报》2014年第5期786-790,849,共6页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】观察清咽合剂的利咽、抗炎和止咳作用。【方法】采用体积分数15%氨水喷雾复制SD大鼠急性咽炎模型观察清咽合剂利咽作用,采用二甲苯致小鼠耳肿胀法复制小鼠炎症模型观察清咽合剂抗炎作用,采用浓氨水喷雾引咳小鼠法观察清咽合剂止咳作用。【结果】(1)利咽作用:空白对照组均无炎症细胞浸润;模型组均见有中至重度炎症细胞浸润;阳性对照组10例中有6例无炎症细胞浸润、4例见少量炎症细胞浸润;清咽合剂低剂量组有1例无炎症细胞浸润、9例见少量炎症细胞浸润;清咽合剂中、高剂量组均有4例无炎症细胞浸润、6例见少量炎症细胞浸润。清咽合剂中、高剂量组可显著减轻炎症细胞浸润(均P<0.01),且与阳性对照药桂林西瓜霜效果相仿。(2)抗炎作用:阳性对照组抑制率为69.8%;清咽合剂低、中、高剂量组抑制率分别为27.3%、60.4%、60.0%。清咽合剂中、高剂量组对二甲苯所致小鼠耳廓肿胀有显著抑制作用(均P<0.01),且与阳性对照组效果相仿,差异无统计学意义(P>0.05)。(3)止咳作用:阳性对照组与清咽合剂低、中、高剂量组镇咳率分别为42.6%、139.0%、64.3%、104.0%。清咽合剂各剂量组均可显著延长小鼠咳嗽潜伏期和减少咳嗽次数,与模型对照组比较,差异均有统计学意义(P<0.05或P<0.01)。【结论】清咽合剂具有利咽、抗炎和止咳的作用,临床上可用于治疗急慢性咽炎及由其所致的咳嗽患者。Objective To investigate the sore-throat-relieving, anti-inflammation and antitussive actions of Qingyan mixture on the animal models. Methods Sore-throat-relieving action of Qingyan mixture was observed in SD rats with acute pharyngitis induced by spray of ammonia water at volume fraction of 15%. Anti-inflammation action of Qingyan mixture was observed on mice model of xylene-induced ear edema, and antitussive action of Qingyan mixture was carried out in mice with cough induced by concentrated ammonia spray. Results （1） The results of sore-throat-relieving action of Qingyan mixture showed that the infiltration of inflammatory cells did not happen in the blank control group, but moderate/severe inflammatory cell infiltration was shown in the model group. In the positive group, 4 rats had slight inflammatory cell infiltration while the left 6 rats had no inflammatory cell infiltration. Low-dose Qingyan mixture group had no inflammatory cell infiltration in one rat, and had slight infiltration in 9 rats. In middle- and high-dose Qingyan mixture groups, 4 rats had no infiltration and 6 had slight infiltration, inflammatory cell infiltration was markedly relieved （P〈0.01） and the sore- throat-relieving action in both groups was similar to the positive medicine of Guilin mirabilitum praeparatum （P〉 0.05）. （2） The anti-inflammation rate was 69.8% in the positive control group, and was 27.3%, 60.4%, 60.0% in low-, middle- and high-dose Qingyan mixture groups respectively. The inhibitory effect of middle- and high-dose Qingyan mixture on xylene-induced mice ear edema was similar to that of the positive control group （P〉0.05） . （3） The cough-relieving rate was 42.6%, 139,0%, 64.3%, 104.0% in positive control group, and low-, middle- and high-dose Qingyan mixture groups respectively. Mice cough latent was obviously prolonged and cough frequency was decreased in the three Qing？/an mixture groups, the differences being significant compared with the model group （P〈0.05 or P〈0.01） . Conc

关 键 词：清咽合剂/药理学 急性咽炎/中药疗法 慢性咽炎/中药疗法 咽/病理学 疾病模型 动物 大鼠 小鼠 

分 类 号：R285.5[医药卫生—中药学]