分光光度法测定新鲜蔬菜中维生素C的含量  

Determination of Contents of Vitamin C in Fresh Vegetables by UV Spectrophotometry

作　　者：李占清[1] 魏海英[1] 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002

出　　处：《中国无机分析化学》2014年第3期79-81,共3页Chinese Journal of Inorganic Analytical Chemistry

基　　金：河北省科技厅科技支撑项目(112764351)资助

摘　　要：水溶性的维生素C是一种人体必需的营养物质.维生素C是含有烯二醇基的多羟基内酯结构,利用其水溶液在波长265 nm处有较强烈的紫外吸收峰,建立了直接用紫外分光光度法测定新鲜蔬菜中维生素C含量的方法.实验结果表明,维生素C的含量在1.0～12.0 μg/mL内线性关系良好,线性方程为A=0.066 03c＋0.019 74（R2 =0.997 51）,检出限为0.044 μg/mL,样品加标回收率为97.5％～105.4％.方法操作简单,准确度高,对新鲜蔬菜的测定结果令人满意.Soluble vitamin C （Vc） is an essential nutrient for human beings. Vc contains enediol moiety in a polyhydroxy lactone structure A spectrophotometric method for the direct determination of Ve content in fresh vegetables was established, because Vc aqueous solution has relative strong characteristic absorbance at 265 nm. A good linear relationship was obtained in the range of 1.0- 12.0 μg/mL of the Vc content. The regression equation was A=0. 066 03c＋0. 019 74 （R2 =0. 997 51）. The detection limit was 0. 044 μg/mL with the recoveries of 97.5% - 105.4%. The method was simple and accurate. It can meet the requirements for the determination of Vc in fresh vegetable samples with satisfactory results.

关 键 词：新鲜蔬菜 维生素C 紫外分光光度法 

分 类 号：O657.32[理学—分析化学] TH744.122[理学—化学]