胃动素对大鼠下丘脑PVN神经元细胞电压依赖性钾电流的影响  

The Effects of Motilin on Potassium Current in Hypothalamic PVN Neurons of Rats

作　　者：王洪忠 李莉[2] 韩波 刘凯 鹿勇[2] 

机构地区：[1]山东省潍坊市潍城区人民医院,山东潍坊261021 [2]菏泽医学专科学校 [3]山东省章丘第二人民医院

出　　处：《中国医学创新》2014年第25期13-16,共4页Medical Innovation of China

基　　金：山东省高等学校科技计划项目(J11LC02)

摘　　要：目的：研究胃动素对大鼠下丘脑PVN神经元细胞电压依赖性钾电流的影响。方法：采用急性分离新生Wistar大鼠下丘脑细胞和全细胞膜片钳技术的方法，观察胃动素对大鼠下丘脑PVN神经元细胞电压依赖性钾电流的影响。结果：胃动素可抑制大鼠下丘脑PVN神经元电压依赖性钾电流，使电流由（5.406±0.86）nA降至（3.621±0.78）nA，差异有统计学意义（P〈0.05）。步进电刺激下丘脑神经元，随着刺激电压强度的增强，钾外向电流增加，使电压-电流曲线（Ⅰ-Ⅴ曲线）右移，但不改变Ⅰ-Ⅴ曲线的形态。结论：胃动素可通过抑制大鼠下丘脑PVN神经元细胞电压依赖性钾电流，发挥其增强胃肠运动的作用。Objective：To study the influence of motilin on the voltage-dependent K＋ current of hypothalamic PVN neurons.Method：Patch clamp experiment：rat hypothalamic PVN neurons cells were acute dissociated and observed the influence of motilin on voltage-dependent K＋ current of hypothalamic PVN neurons using whole-cell patch-clamp techniques.Result：Motilin could inhibit the voltage-dependent K＋ current of rat hypothalamic PVN neurons.The current was decreased from （5.406±0.86）nA to （3.621±0.78）nA,the difference was statistically significant（P〈0.05）. Step electric stimulation of hypothalamic neurons,with stimulus voltage strength increased,outward potassium current increased,and the Ⅰ-Ⅴ curve shifted right,but the form of the Ⅰ-Ⅴ curve was not changed.Conclusion：Motilin can enhance the motility of gastrointestinal through inhibiting the voltage-dependent K＋ current of hypothalamic PVN neurons.

关 键 词：胃动素 下丘脑PVN细胞 全细胞膜片钳技术 电压依赖性钾电流 

分 类 号：R965[医药卫生—药理学]