淫羊藿苷对受损斑马鱼胚胎血管发生的促进作用  

Effect of icariin on the angiogenesis of impaired- vessel of zebra fish embryo

作　　者：李菲[1] 黄晓慧[2] 龚其海[1] 李铭源[2] 董红心[1,3] 石京山[1] 

机构地区：[1]遵义医学院药理学教研室暨基础药理省部共建教育部重点实验室,贵州遵义563099 [2]澳门大学中国医学科学研究院,澳门000853 [3]美国西北大学精神病学与行为科学实验室,美国芝加哥60611

出　　处：《遵义医学院学报》2014年第4期397-399,共3页Journal of Zunyi Medical University

基　　金：973计划前期研究专项(NO:2011CB512009);教育部创新团队项目(NO:IRT1197);遵义医学院博士启动基金项目(NO:F-581)

摘　　要：目的以损伤转基因斑马鱼胚胎为模型研究淫羊藿苷对受损血管发生的影响。方法采用血管内皮生长因子受体抑制剂(VRI)制备斑马鱼胚胎的血管损伤模型,3 h后给予不同浓度(30 100,300μM)的淫羊藿苷处理24、48 h,之后对胚胎体节间血管和肠下静脉进行拍照并计数。结果给予VRI作用3 h后观察到受损血管数量不到10条。3个浓度的淫羊藿苷均可促进受损的斑马鱼血管发生,其促血管发生的作用在48 h时较为明显,100、300μM的淫羊藿苷组体节间血管数量分别为22条和26条左右。结论淫羊藿苷可促进受损的斑马鱼体节间血管和肠下静脉的发生。Objective To investigate the effect of icariin on impaired vessel using impaired transgenic zebra fish embryo model. Methods Zebra fish embryo vessels impaired model was induced by vascular endothelial growth factor receptor inhibitor. Three hours later, these zebra fish embryos were treated with different concentrations of icariin （30, 100, 300μM） for 24 hours or 48 hours, then intersegmental blood vessels and subintestinal veins of embryos were counted and photographed respectively. Results There were less than 10 defective vessels in the group treatment with VRI for 3 h. All three concentrations of icariin could improve the growth of impaired vessels of zebra fish embryos, and the effect was more significance on 48 hours. The number of intersegmental blood vessels in 100 and 300 μM icariin groups were about 22 and 26, respectively. Conclusion Icariin has the angiogenesis - facilitated effect on intersegmental blood vessels and subintestinal veins in the impaired - vessel of zebra fish embryo.

关 键 词：淫羊藿苷 斑马鱼胚胎 血管发生 

分 类 号：R972[医药卫生—药品]