一种电击转化枯草芽孢杆菌方法的优化  

Optimization of a Method about Electro-transformation in Bacillus Subtilis

作　　者：张爽[1] 薛正莲[1] 陈环[1] 苏燕南[1] 王洲[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《重庆理工大学学报（自然科学）》2014年第8期60-63,80,共5页Journal of Chongqing University of Technology：Natural Science

基　　金：安徽省自然科学基金项目(11040606M81);安徽省高校自然科学基金项目(KJ2009A168)

摘　　要：提出了一种电击转化枯草芽孢杆菌的方法。该方法通过调节细胞培养和电击转化过程中培养基和溶液成分来调节细胞渗透压,提高细胞在高电场强度电击后的存活率而达到提高转化效率的目的。优化前的转化率为5.0×105cfu/μg,经优化后转化效率可达8×106cfu/μg,比优化前提高了15倍。This article provides a method of electro-transformation in Bacillus Subtilis. This method is to regulate cell osmotic pressure and solution composition by regulating cell culture and shock in the process of transformation medium,for improving the survival rate after cells in high electric field intensity of electric shock and achieving the goal that improve the transformation efficiency. The transformation rate is 5. 0 × 10^5 cfu /μg before optimizing. After optimizing,the transformation efficiency is up to 8 × 10^6 cfu /μg,being 15 times higher than before optimization. It greatly promotes the conversion efficiency.

关 键 词：枯草芽孢杆菌 电击转化 转化率 

分 类 号：Q785[生物学—分子生物学]