HPLC法测定苦豆草药材中生物碱的含量  

作　　者：陈海燕[1] 冷晓红[1] 郭鸿雁[1] 郭超[1] 

机构地区：[1]宁夏职业技术学院,银川750021

出　　处：《北方药学》2014年第11期2-3,共2页Journal of North Pharmacy

基　　金：科技部国家科技支撑项目(编号2011BAI05B04);苦豆子药材及提取物质量标准研究和生产过程标准研究

摘　　要：目的：建立苦豆草药材中生物碱含量的测定方法。方法：采用HPLC法测定,色谱柱为Agilent TC-C18（2）（250mm×4.6mm,5μm）;流动相为乙腈—0.05mol·L-1磷酸二氢钾水溶液（三乙胺2.0ml/L）,梯度洗脱;流速为1.0ml/min,柱温30℃;检测波长205nm。结果：苦豆草中主要生物碱槐定碱在0.00804mg·ml-1~0.2412mg·ml-1范围内有良好的线性关系,回收率为99.3%,RSD为1.63%;氧化苦参碱在0.00808mg·ml-1~0.2424mg·ml-1范围内有良好的线性关系,回收率为99.4%,RSD为1.82%。结论：该方法简便易行、结果准确、可靠,重复性好,可用于苦豆草中生物碱的定量分析方法。Objective Establish a method Sophora medicinal alkaloid content. Methods by HPLC column was Agilent Zorbax SB-C 18 column （250mm ×4.6mm,5μm）;mobile phase of acetonitrile-0.05mol·L-1 potassium dihydrogen phosphate （triethylamine 2.0ml/L）, gradient elution off;flow 1.0ml/min, column temperature was 30℃;detection wavelength of 205nm. Results The main alkaloid sophoridine a good linear relationship ·ml-1~0.2412mg·ml-1 range of 0.00804mg, recovery was 99.3%, RSD was 1.63%; Oxymatrine at 0.00808mg· ml-1~0.2424mg·ml^-1 has a good linear relationship within the range of recovery was 99.4%, RSD was 1.82%. Conclusion The method is simple, accurate, reproducible and can be used for quantitative analysis of alkaloid methods.

关 键 词：HPLC 苦豆草 生物碱 

分 类 号：R927.2[医药卫生—药学]