HPLC法测定阿司匹林肠溶片中阿司匹林的含量  

Determination the Content of Aspirin in Aspirin Enteric-Coated Tablet by HPLC

作　　者：孙瑛 何春龙[2] 成业[2] 任珊珊[2] 

机构地区：[1]内蒙古伊生泰妇产医院药剂科,呼和浩特010040 [2]内蒙古医科大学药学院,呼和浩特010110

出　　处：《北方药学》2014年第11期12-13,共2页Journal of North Pharmacy

摘　　要：目的:建立阿司匹林肠溶片中阿司匹林的含量测定方法。方法:采用高效液相色谱法测定阿司匹林肠溶片中阿司匹林的含量。色谱柱为Hypersil ODS2(5um,250mm×4.6mm),流动相为甲醇∶0.5%乙酸溶液(37∶63);检测波长:276nm;流速:0.8ml·min-1;柱温:25℃;进样量:10μL。结果:阿司匹林在20μg·ml-1-150μg·ml-1范围内与峰面积积分值呈良好的线性关系,回归方程为y=23290x-9400.2,r=0.9991。平均加样回收率为101.2%,RSD=0.94%。结论:本方法操作简单,重复性好,结果准确,为阿司匹林的质量控制提供了方法。Objective：To establish a RP-HPLC method for the determination of aspirin in enteric-coated aspirin tablet.Methods：The separation was performed on a Hypersil ODS2 column （250mm×4.6mm,5μm）.The mobile phase was methanol∶0.5%acetic acid （37∶63） with a flow rate of 0.8 ml/min. The detection wavelength was at 267nm. Results：Aspirin in the range of 20μL^150μg·ml-1showed a good linear relationship （r= 0.9997）.The average recovery was 118.18% and RSD was 1.44%（n=6）.Conclusion：This accurate and reliable HPLC method could be used for the determination of aspirin.

关 键 词：阿司匹林 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]