WF16616的加压毛细管电色谱法测定  

Determination of WF16616 by Pressurized Capillary Electrochromatography

作　　者：赵琪[1] 张长清[1] 胡海峰[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2014年第9期873-875,共3页Chinese Journal of Pharmaceuticals

基　　金：国家重大科学仪器设备开发专项(2011YQ15007208);国家“重大新药创新”科技重大专项(2012ZX09301002-003)

摘　　要：建立了加压毛细管电色谱法快速测定脂肽类抗真菌化合物WF16616。流动相为乙腈∶6.5 mmol/L磷酸盐缓冲液（pH 5.2）（48∶52）,流速0.08 ml/min,检测波长212 nm,分离电压11 kV。WF16616在0.06--1.60 mg/ml范围内线性关系良好。平均回收率为101.17%,RSD为3.28%。A pressurized capillary electrochromatography （pCEC） method was established for the determination of WF16616, an antifungal lipopeptide compound. The method was conducted with the mobile phase of acetonitrile ： 6.5 mmol/L phosphate buffer （pH 5.2） （48 ： 52） at the flow rate of 0.08 ml/min, the detection wavelength of 212 nm, and the separation voltage of 11 kV. The calibration curve for WF 16616 was linear in the concentration range of 0.06 - 1.60 mg/ml. The average recovery was 101.17%, with RSD of 3.28%.

关 键 词：WF16616 抗真菌药 米卡芬净 加压毛细管电色谱 测定 

分 类 号：R917[医药卫生—药物分析学] TQ460.72[医药卫生—药学]