DNA条形码技术在陕西省鼠疫疫区宿主动物鉴定中的应用  

Identification on host animals for plague by DNA barcoding technology in Shaanxi province

作　　者：安翠红[1] 孙养信[1] 陈宝宝[1] 范锁平[1] 霍丽霞[1] 佘建军[1] 吕文[1] 

机构地区：[1]陕西省疾病预防控制中心鼠布生防科,西安710054

出　　处：《中华流行病学杂志》2014年第9期1042-1045,共4页Chinese Journal of Epidemiology

基　　金：陕西省科学技术研究发展计划（2012K16-12-03）

摘　　要：目的 探讨DNA条形码技术在鼠疫疫区宿主动物鉴定中的应用,并建立陕西省鼠疫疫区宿主动物DNA条形码数据库.方法 运用DNA条形码技术检测陕西省定边县鼠疫疫区3目6科12属14种139只宿主动物以及7个鼠种8个不同部位的62份残体标本线粒体细胞色素C氧化酶Ⅰ亚基基因序列.结果 种内遗传距离≤2％,种间遗传距离为8.9％～ 15.1％;邻接法系统树能区分14个不同物种的类群;采集的残体标本能检测到目的基因并用于鉴定;研究中还纠正了既往定边县鼠疫疫区黄鼠命名.结论 DNA条形码技术能弥补形态学鉴定的不足,可用于鼠疫疫区宿主动物及残体标本的分类鉴定.Objective To apply the DNA barcoding technology for identification on host animal and to establish the host animal DNA bar code database on natural foci of plague in Shaanxi.Methods 139 host animals belonging to 3 orders,6 families and 12 genera and 62 residues belonging to 7 species from 8 different parts of the province,were detected.DNA barcoding technology was used to analyze the DNA CO Ⅰ gene sequence on the natural foci of plague in Dingbian county.Results The intra-specific genetic distance was less than 2% while the inter-specific distance ranged from 8.9% to 15.1%.Fourteen major clusters were apparently showed on a Neighbor-Joining tree.Residue samples could be detected regarding the objective gene.Alashan ground squirrel was previously noticed to carry 14 major clusters,which were previously mistakenly named as Citellus dauricus in Dingbian county.Conclusion DNA barcoding technology could overcome the shortcomings caused by the morphological identification so could be used to identify the host animal and residues in the natural focus of plague.

关 键 词：鼠疫疫区 DNA条形码 基因 线粒体细胞色素C氧化酶Ⅰ亚基 鼠种鉴定 残体鉴定 

分 类 号：R516.8[医药卫生—内科学]