穿心莲内酯对白念珠菌生物膜分散的影响  

Effect of andrographolide on Candida albicans biofilm dispersion

作　　者：施高翔[1,2] 严园园[1,2] 邵菁[1,2] 陆克乔 张梦翔[1,2] 汪天明[1,2] 汪长中[1,2] 

机构地区：[1]安徽中医药大学中西医结合临床学院,安徽合肥230038 [2]安徽省中医药科学院中西医结合研究所,安徽合肥230038

出　　处：《中国中药杂志》2014年第17期3339-3343,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073127);安徽中医药大学科研项目(2013ZR009;2013RC001)

摘　　要：随着真菌感染日渐增多,白念珠菌的防治成为当前抗真菌感染的重点。该研究拟探讨中药单体穿心莲内酯(AG)对白念珠菌生物膜分散的影响。实验以微孔板结合医用导管片构建白念珠菌生物膜模型,分别通过XTT减低法和平板法发现1 000,500,250 mg·L^(-1)AG能不同程度的影响白念珠菌生物膜分散细胞的活性;以扫描电镜观察导管片残余生物膜形态结构,发现1 000,500,250 mg·L^(-1)AG能剂量依赖性的诱导白念珠菌生物膜的分散,且分散出的细胞以酵母相为主;实时荧光定量PCR(qRT-PCR)法检测发现AG使HSP90表达上调,使UME6和PES1表达下调。该研究表明AG能一定程度的诱导白念珠菌生物膜分散。Along with the increase in fungal infections, Candida albicans prevention and control become the focus of anti-fun- gal infection at present. This study aims to discuss the effect monomer andrographolide （AG） on C. albicans biofilm dispersion. In the experiment, micro-well plates and medical catheter pieces were used to establish the C. albicans biofilm model. It was discovered by XTT assay and flat band method that 1 000, 500,250 mg · L-1 AG could impact the activity of C. albicans biofilm dispersion cells. The morphological structures of residual biofilms on catheter pieces were observed with scanning electron microscopy, which showed that 1 000, 500,250 mg·L-1 AG could induce C. albicans biofilm dispersion in a dose-dependent manner, and the dispersed cells were dominated by the yeast phase. According to the real-time fluorescence quantification PCR （qRT-PCR） test, AG could up-regulate HSP90 expression and down-regulate UME6 and PES1 expressions. This study demonstrates that AG could induce C. albicans biofilm dispersion to some extent.

关 键 词：穿心莲内酯 白念珠菌 生物膜 分散 

分 类 号：R285[医药卫生—中药学]