宁冈芙蓉李与福建芙蓉李的ISSR分析  

ISSR Analysis of Furong Plums from Ninggang and Fujian

作　　者：方智振[1] 叶新福[1] 周丹蓉[1] 姜翠翠[1] 廖汝玉[1] 潘少霖[1] 

机构地区：[1]福建省农业科学院果树研究所,福州350013

出　　处：《中国南方果树》2014年第5期12-14,共3页South China Fruits

基　　金：福建省农业科学院青年科技人才创新基金项目(2012DBS-6);福建省科技重大专项专题(2013NZ0002-1);农业部公益类行业科研专项(201003058-8);福建省财政专项(CXTD2011-19)资助

摘　　要：采用ISSR分子标记技术对宁冈芙蓉李与福建芙蓉李的遗传差异进行分析。从32条引物中筛选出10条引物对4个芙蓉李材料的DNA序列进行PCR扩增,共得到62个条带,多态性条带17个;宁冈芙蓉李与福建芙蓉李的ISSR指纹图谱差异显著,来自于不同地方的福建芙蓉李的ISSR指纹图谱之间无显著性差异。引物UBC813、UBC818、BC825、UBC840、UBC844和UBC856均可以将宁冈芙蓉李与福建芙蓉李区分开。Genetic differences between Furong plums from Ninggang and Fujian were detected by ISSR molecular markers. A total number of 62 bands were amplified using 10 primers, of which 17 bands were polymorphic. Significant differences were detected between ISSR fingerprintings of Furong plums from Ninggang and Fujian. There were no significant differences among Furong plums from different places of Fujian. Furong plums from Ninggang and Fujian could be distinguished by primers UBC813, UBC818, UBC25, UBC40, UBC44 and UBC56.

关 键 词：芙蓉李 ISSR 遗传差异 

分 类 号：F426.8[经济管理—产业经济]