人参皂苷Rg1抗肝纤维化的体视学分析  

A stereological analysis of ginsenoside Rg1 in inhibiting hepatic fibrosis

作　　者：董向前[1] 段丽平[1] 缪应雷[1] 梁兵[1] 李树安[1] 柳波[2] 詹尔益[2] 宋精玲[3] 马岚青[1] 

机构地区：[1]昆明医科大学第一附属医院消化内科,昆明650032 [2]昆明医科大学重点实验室,昆明650032 [3]昆明医科大学电镜室,昆明650032

出　　处：《重庆医学》2014年第27期3599-3601,3616,共4页Chongqing medicine

基　　金：昆明医学院青年基金(kylc200510)

摘　　要：目的研究三七总甙单体成分人参皂苷Rg1对肝纤维化的作用。方法采用50%Ccl4造大鼠肝纤维化模型,共35d,同时予三七总皂甙单体成分人参皂苷Rg1不同剂量治疗,于第5周(实验结束)时分离肝组织,光镜和电镜观察肝组织病理变化,并应用体视学方法计量各组大鼠肝细胞线粒体的体积密度(Vvm)、面积密度(Svm)、比表面(Qm)、面数密度(Nam)。结果各组大鼠肝细胞线粒体体视学结果比较组间存在差异。三七总甙组、Rg1低剂量组和等渗盐水组Vvm较正常对照组显著增高,差异有统计学意义(P<0.01);Rg1高剂量组、Rg1中剂量组和秋水仙碱组Vvm较正常对照组呈增高趋势,但差异无统计学意义(P>0.05);Rg1高、中、低剂量组,三七总甙组、秋水仙碱组Vvm与等渗盐水组比较呈降低趋势,但差异无统计学意义(P>0.05)。Rg1低剂量组、三七总甙组、秋水仙碱组、等渗盐水组Svm较正常对照组呈显著增高,差异有统计学意义(P<0.01);Rg1高、中剂量组、三七总甙组、秋水仙碱组Svm较等渗盐水组显著降低,差异有统计学意义(P<0.01);Rg1高剂量组Svm较Rg1低剂量组明显降低,差异有统计学意义(P<0.05)。Rg1低剂量组、秋水仙碱组、等渗盐水组Nam较正常对照组显著增高,差异有统计学意义(P<0.01);Rg1高、中剂量组,三七总甙组Nam较等渗盐水组显著降低,差异有统计学意义(P<0.01);Rg1高剂量组Nam较Rg1低剂量组明显降低,差异有统计学意义(P<0.05)。各组Qm较正常对照组呈降低趋势,但差异有统计学意义(P>0.05)。结论人参皂苷Rg1具有三七总甙的抗肝纤维化作用,在一些方面甚至超过三七总甙,且Rg1的上述作用与剂量呈正相关,故认为人参皂苷Rg1是较理想的防治肝纤维化的药物。Objective To investigate the effect of Panax notoginsenosides monomers ginsenoside Rg 1 in inhibiting hepatic fibro-sis .Methods The rat model of hepatic fibrosis was established by using 50% Ccl4 ,total 35 d .The different doses of Rg1was ad-ministered by hypodermical injection .At the end of the treatment ,the pathological changes of hepatic tissue were observed by light and transmission electron microscope .The stereological method was adopted to measure the volume density （Vvm） ,area density （Svm） ,specific surface（Qm） and surface number density （Nam） of liver cell mitochondria in various groups .Results The stereo-logical data of liver cell mitochondria showed that the statistical differences existed among various groups .Vvm in the Panax Notog-insenosides ,low dose Rg1 and isotonic saline groups were significantly increased compared with the normal control group with sta-tistical difference（P〈0 .01）;Vvm in the high dose Rg1 ,middle dose Rg1 and colchicine groups showed the increasing trend com-pared with the normal control group without statistical difference （P〉0 .05）;Vvm in the high ,middle and low dose Rg1 ,Panax No-toginsenosides and colchicine groups showed the decreasing trend compared with the isotonic saline group without statistical differ-ence（P〉0 .05） .Svm in the low dose Rg1 ,Panax Notoginsenosides ,colchicine and isotonic saline groups were significantly increased compared with the normal group with statistical difference （P〈0 .01）;Svm in the high dose Rg1 ,middle dose Rg1 ,Panax Notogin-senosides and colchicine groups was significantly induced compared with the isotonic saline group with statistical difference （P〈0 .01）;Svm in the high dose Rg1 was reduced compared with the middle dose Rg1 group（P〈0 .05） .Nam in the low dose Rg1 ,col-chicine and isotonic saline group was significantly increased compared with the normal group （P〈0 .01）;Nam in the high dose Rg1 , middle dose Rg1 and Panax Notoginsenosides groups were significantly reduced

关 键 词：肝硬化 三七皂甙 人参皂苷RG1 体视学 肝星状细胞 

分 类 号：R285.5[医药卫生—中药学]