长春花黄素单加氧酶基因的克隆与组织表达分析  

Cloning and Tissue Expression of Flavin Monooxygenase YUC Gene from Catharanthus roseus

作　　者：许菲[1] 张颖[1] 潘琪芳[1] 唐克轩[1] 赵静雅[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2014年第4期1-7,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家高技术研究发展计划(863)(2011AA100605)

摘　　要：利用RACE方法克隆到长春花中编码黄素单加氧酶的基因(YUCCA),YUCCA(YUC)是植物生长素吲哚乙酸(IAA)合成通路上的关键酶。长春花YUC(CrYUC,GeneBank登陆号KF827426)cDNA序列全长1 863bp,包括编码405个氨基酸的1 218bp开放阅读框、5’非编码区、3’非编码区和poly A尾巴;其相应的DNA中有3个内含子。生物信息学分析结果显示:预测CrYUC的分子量为45.3kDa,等电点为9.01;有16个磷酸化活性位点;与其他物种的YUC有较高相似度。初步表达分析发现:YUC在长春花的叶片、花、根及茎中均有表达,且茎及叶片中表达量高于花及根中表达量。In this study, the YUCCA gene which encodes flavin monooxygenase （named as CrYUC, GeneBank number：KF827426）was cloned from Catharanthus roseus. YUC plays an important role in the auxin biosynthesis. The full length of CryuC cDNA is 1 863 bp including 1 218 bp open reading frame which encodes 405 amino acids, 3 ~, 5＇-unencoding area and Poly A tail area. The DNA sequence of CryuC contains three introns. The results of biovinformation analysis showed that the molecular weight of CryuC was 45. 3 and the isoelectric point was 9. 01. There was 16 phosphorylation activity sites and CrYUC was homologous with YUCs of other plant species. The RT-qPCR result showed that CrYUC mRNA existed in leaf,flower,stem and root. But the expression level of stem and leaf were higher than that of root and flower.

关 键 词：长春花 黄素单加氧酶基因 吲哚乙酸合成途径 基因克隆 组织表达分析 

分 类 号：Q943[生物学—植物学]