仙人掌多糖对人肺癌A549细胞形态结构的影响  

The influence of shape and structure in human lung cancer A549 cell by purification of Cactus polysaccharides

作　　者：车加祥 邵淑丽[1] 徐君懿 陈闯[1] 张伟伟[1] 徐兴军[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2014年第5期68-70,共3页Journal of Science of Teachers＇College and University

基　　金：黑龙江省自然科学基金项目(C200624;C201241)

摘　　要：以人肺癌A549细胞为研究对象,探讨仙人掌多糖组分OP1对人肺癌A549细胞生长抑制,以及对其形态、结构的影响.采用台盼蓝拒染法测定人肺癌A549细胞生长抑制曲线,通过倒置显微镜观察不同质量浓度的仙人掌多糖组分OP1作用于人肺癌A549细胞引发的形态变化.结果表明,仙人掌多糖组分OP1能抑制人肺癌A549细胞增殖,诱导人肺癌A549细胞凋亡,在一定范围内,呈时间、剂量依赖性,作用48h的IC50为(597.55±28.97)μg/mL.经仙人掌多糖组分OP1诱导后,人肺癌A549细胞形态结构出现典型的凋亡特征.Study to human lung cancer A549 cells as the research object, explore purification OP1 of Cactus polysaccharides on morphological structure of human lung cancer A549 cells. The inhibitory ratio of cells was measured by trypan blue stain assay. By inverted microscope to observe different concentrations of purification OP1 of cactus polysaccharides to act on human lung cancer A549 cell morphological changes. The result shown that purification OP1 of Cactus polysaccharides can inhibit proliferation of human lung cancer A549, and induce apoptosis of human lung cancer A549, and in a range of time, dose-dependent, the ICs0 is （ 597.55 ± 28.97 ） μg/mL. Purification OP1 of Cactus polysaccharides can make human lung cancer A549 DNA fragmentation.

关 键 词：仙人掌多糖组分OP1 人肺癌A549细胞 细胞凋亡 

分 类 号：Q28[生物学—细胞生物学]